95158

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 141791..142434 Replicon plasmid pKPC2_095649
Accession NZ_CP026584
Organism Klebsiella pneumoniae strain WCHKP649

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag BT120_RS01185 Protein ID WP_001044770.1
Coordinates 142018..142434 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag BT120_RS01180 Protein ID WP_001261282.1
Coordinates 141791..142021 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BT120_RS01145 137014..137793 - 780 WP_013214009.1 site-specific integrase -
BT120_RS01150 137977..138981 - 1005 WP_011977814.1 hypothetical protein -
BT120_RS01155 139011..139214 - 204 WP_011977813.1 hypothetical protein -
BT120_RS01160 139260..139781 - 522 WP_013214008.1 hypothetical protein -
BT120_RS01165 139839..140189 - 351 WP_032738650.1 hypothetical protein -
BT120_RS01170 140268..141212 - 945 WP_011977810.1 hypothetical protein -
BT120_RS01175 141373..141807 - 435 WP_015493090.1 hypothetical protein -
BT120_RS01180 141791..142021 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BT120_RS01185 142018..142434 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
BT120_RS01190 142508..144070 + 1563 WP_004206609.1 AAA family ATPase -
BT120_RS01195 144055..145077 + 1023 WP_000361404.1 helicase UvrD -
BT120_RS01200 145333..146030 + 698 Protein_215 IS1 family transposase -
BT120_RS01205 146059..146331 + 273 Protein_216 IS5/IS1182 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaCTX-M-65 / blaTEM-1B / rmtB / catA2 - 1..156099 156099


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T95158 WP_001044770.1 NZ_CP026584:142018-142434 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T95158 NZ_CP026584:142018-142434 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT95158 WP_001261282.1 NZ_CP026584:141791-142021 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT95158 NZ_CP026584:141791-142021 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References