Detailed information of TA system TA095157

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 2364..2838
• Replicon: plasmid p2_095649
• Accession: NZ_CP026583
• Organism: Klebsiella pneumoniae strain WCHKP649

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: BT120_RS00100
• Protein ID: WP_004197762.1
• Coordinates: 2572..2838 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: BT120_RS00095
• Protein ID: WP_000879771.1
• Coordinates: 2364..2588 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BT120_RS00080	449..994	+	546	Protein_1	nuclease	-
BT120_RS00085	969..1397	+	429	Protein_2	mobilization protein	-
BT120_RS00090	1485..1697	+	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
BT120_RS00095	2364..2588	+	225	WP_000879771.1	TraY domain-containing protein	Antitoxin
BT120_RS00100	2572..2838	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BT120_RS00105	2908..3258	-	351	WP_004197756.1	hypothetical protein	-
BT120_RS00110	3324..3869	-	546	WP_023317533.1	hypothetical protein	-
BT120_RS00115	3895..4274	-	380	Protein_8	hypothetical protein	-
BT120_RS00120	4962..5192	+	231	Protein_9	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T95157 WP_004197762.1 NZ_CP026583:2572-2838 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T95157 NZ_CP026583:2572-2838 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT95157 WP_000879771.1 NZ_CP026583:2364-2588 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT95157 NZ_CP026583:2364-2588 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Antitoxin

• AlphaFold DB: S1EJF5