Detailed information of TA system TA095154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 3685526..3686175
• Replicon: chromosome
• Accession: NZ_CP026581
• Organism: Proteus mirabilis isolate GN2

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B4EZB9
• Locus tag: C3940_RS17095
• Protein ID: WP_012368534.1
• Coordinates: 3685526..3685945 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: B4EZC0
• Locus tag: C3940_RS17100
• Protein ID: WP_012368535.1
• Coordinates: 3685942..3686175 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3940_RS17055	3680805..3681683	-	879	WP_017628653.1	23S rRNA pseudouridine(2604) synthase RluF	-
C3940_RS17060	3681859..3682773	-	915	WP_004249085.1	fatty acid biosynthesis protein FabY	-
C3940_RS17065	3682797..3683234	-	438	WP_004246810.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C3940_RS17070	3683315..3683932	-	618	WP_004246809.1	glucose-1-phosphatase	-
C3940_RS17085	3684584..3685000	-	417	WP_046334846.1	hypothetical protein	-
C3940_RS17090	3684978..3685301	-	324	WP_104731870.1	hypothetical protein	-
C3940_RS17095	3685526..3685945	-	420	WP_012368534.1	type II toxin-antitoxin system VapC family toxin	Toxin
C3940_RS17100	3685942..3686175	-	234	WP_012368535.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C3940_RS18830	3686442..3686594	+	153	WP_172764486.1	hypothetical protein	-
C3940_RS17105	3687557..3688765	+	1209	WP_060555750.1	DUF262 domain-containing protein	-
C3940_RS17110	3688982..3689203	-	222	WP_060555751.1	hypothetical protein	-
C3940_RS17115	3689251..3689538	+	288	WP_104731871.1	transposase	-
C3940_RS17120	3689988..3690467	+	480	WP_060555753.1	Hcp family type VI secretion system effector	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (9-56)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15371.88 Da
• Isoelectric Point: 7.7288

>T95154 WP_012368534.1 NZ_CP026581:c3685945-3685526 [Proteus mirabilis]
VKKVYMLDTNICSFIMREQPISLLEKLQKCVMNHDTIVISAITYSEMRFGAIGKKASPKHNRLVDAFCERVDAILAWDKA
AVDATTVIKKCLSDVGLPIGNNDSAIAGHAVAVNAILVTNNTREFSRVEGLKIEDWTHV

Nucleotide

• Length: 420 bp

>T95154 NZ_CP026581:c3685945-3685526 [Proteus mirabilis]
GTGAAAAAAGTCTATATGCTTGATACAAACATTTGTTCTTTTATCATGCGTGAGCAACCTATTTCTTTGCTCGAAAAACT
TCAGAAATGTGTAATGAACCACGATACTATTGTCATATCAGCGATTACTTATTCAGAGATGCGCTTTGGAGCAATAGGAA
AAAAAGCATCACCTAAACACAATCGTTTGGTTGATGCTTTCTGTGAACGCGTTGACGCGATTTTGGCATGGGATAAAGCG
GCGGTAGACGCAACTACCGTGATAAAAAAATGCTTATCTGATGTGGGATTGCCCATAGGAAATAATGATAGCGCGATTGC
TGGTCATGCTGTCGCGGTTAATGCAATATTGGTAACAAATAATACCCGTGAATTTTCACGAGTTGAAGGACTAAAAATAG
AGGACTGGACTCATGTTTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8890.06 Da
• Isoelectric Point: 4.4870

>AT95154 WP_012368535.1 NZ_CP026581:c3686175-3685942 [Proteus mirabilis]
MRTRTVAVFKNGSNRAIRLPKDFDFDGVNELEISRDGDCIILRPVKPNWISLQNEEKADSDFLLERDDVIEEGRVIL

Nucleotide

• Length: 234 bp

>AT95154 NZ_CP026581:c3686175-3685942 [Proteus mirabilis]
ATGAGAACGAGAACTGTAGCTGTATTTAAAAACGGAAGTAACCGAGCCATTCGCTTACCTAAAGATTTCGATTTTGATGG
TGTCAATGAACTCGAGATAAGCCGAGATGGGGATTGTATTATTTTACGCCCTGTAAAACCTAACTGGATTTCATTACAAA
ACGAAGAAAAAGCCGACAGTGATTTCTTGCTTGAACGCGATGATGTTATTGAAGAAGGACGAGTTATTCTGTGA

Structures

Toxin

• AlphaFold DB: B4EZB9

Antitoxin

• AlphaFold DB: B4EZC0