Detailed information of TA system TA095146

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3652143..3652837
• Replicon: chromosome
• Accession: NZ_CP026580
• Organism: Escherichia coli strain WCHEC005237

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: C4E05_RS19840
• Protein ID: WP_001094398.1
• Coordinates: 3652143..3652511 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: C4E05_RS19845
• Protein ID: WP_077249034.1
• Coordinates: 3652532..3652837 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4E05_RS19820	3647316..3648272	+	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-
C4E05_RS19825	3648269..3650467	+	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
C4E05_RS19830	3650477..3651433	+	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
C4E05_RS19835	3651412..3651822	+	411	WP_001111348.1	hypothetical protein	-
C4E05_RS19840	3652143..3652511	-	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
C4E05_RS19845	3652532..3652837	-	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C4E05_RS19850	3652941..3653585	-	645	WP_000086755.1	hypothetical protein	-
C4E05_RS19855	3653604..3653825	-	222	WP_000692345.1	DUF987 domain-containing protein	-
C4E05_RS19860	3653888..3654364	-	477	WP_001186774.1	RadC family protein	-
C4E05_RS19865	3654380..3654853	-	474	WP_000855059.1	antirestriction protein	-
C4E05_RS19875	3655195..3656016	-	822	WP_001234565.1	DUF945 domain-containing protein	-
C4E05_RS19885	3656117..3656325	-	209	Protein_3412	DUF905 family protein	-
C4E05_RS19890	3656426..3656881	-	456	WP_000581506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3625653..3666480	40827

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T95146 WP_001094398.1 NZ_CP026580:c3652511-3652143 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T95146 NZ_CP026580:c3652511-3652143 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
AACTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT95146 WP_077249034.1 NZ_CP026580:c3652837-3652532 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT95146 NZ_CP026580:c3652837-3652532 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4