Detailed information of TA system TA095134

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1873382..1874217
• Replicon: chromosome
• Accession: NZ_CP026580
• Organism: Escherichia coli strain WCHEC005237

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1X9TM58
• Locus tag: C4E05_RS11130
• Protein ID: WP_000854761.1
• Coordinates: 1873382..1873759 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: C4E05_RS11135
• Protein ID: WP_001295723.1
• Coordinates: 1873849..1874217 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4E05_RS11090	1868771..1868953	-	183	WP_001016348.1	hypothetical protein	-
C4E05_RS11100	1869407..1869796	-	390	WP_001445118.1	IS110 family transposase	-
C4E05_RS11105	1870290..1870694	+	405	WP_000839179.1	IS66 family insertion sequence hypothetical protein	-
C4E05_RS11110	1870691..1871038	+	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
C4E05_RS11115	1871087..1872622	+	1536	WP_001333339.1	IS66-like element ISEc22 family transposase	-
C4E05_RS11120	1873057..1873170	-	114	WP_001161660.1	DUF957 domain-containing protein	-
C4E05_RS11125	1873183..1873385	-	203	Protein_1725	hypothetical protein	-
C4E05_RS11130	1873382..1873759	-	378	WP_000854761.1	TA system toxin CbtA family protein	Toxin
C4E05_RS11135	1873849..1874217	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C4E05_RS11140	1874380..1874601	-	222	WP_000692341.1	DUF987 domain-containing protein	-
C4E05_RS11145	1874664..1875140	-	477	WP_001186774.1	RadC family protein	-
C4E05_RS11150	1875156..1875629	-	474	WP_000855059.1	antirestriction protein	-
C4E05_RS11160	1875971..1876789	-	819	WP_001234729.1	DUF945 domain-containing protein	-
C4E05_RS11170	1876944..1877102	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14205.25 Da
• Isoelectric Point: 7.3249

>T95134 WP_000854761.1 NZ_CP026580:c1873759-1873382 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T95134 NZ_CP026580:c1873759-1873382 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT95134 WP_001295723.1 NZ_CP026580:c1874217-1873849 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT95134 NZ_CP026580:c1874217-1873849 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TM58

Antitoxin

• AlphaFold DB: S1NM52