Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 930070..930724 Replicon chromosome
Accession NZ_CP026580
Organism Escherichia coli strain WCHEC005237

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag C4E05_RS06795 Protein ID WP_000244777.1
Coordinates 930317..930724 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C4E05_RS06790 Protein ID WP_000354046.1
Coordinates 930070..930336 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4E05_RS06765 925239..925982 + 744 WP_000951964.1 SDR family oxidoreductase -
C4E05_RS06770 926039..927472 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
C4E05_RS06775 927517..927828 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
C4E05_RS06780 927992..928651 + 660 WP_000250274.1 hemolysin III family protein -
C4E05_RS06785 928847..929827 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
C4E05_RS06790 930070..930336 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C4E05_RS06795 930317..930724 + 408 WP_000244777.1 toxin CptA Toxin
C4E05_RS06800 930764..931285 - 522 WP_001055874.1 flavodoxin FldB -
C4E05_RS06805 931397..932293 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C4E05_RS06810 932318..933028 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C4E05_RS06815 933034..934767 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T95131 WP_000244777.1 NZ_CP026580:930317-930724 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T95131 NZ_CP026580:930317-930724 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT95131 WP_000354046.1 NZ_CP026580:930070-930336 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT95131 NZ_CP026580:930070-930336 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References