Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 93113..93638 Replicon plasmid pRmtB1_005237
Accession NZ_CP026579
Organism Escherichia coli strain WCHEC005237

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag C4E05_RS02165 Protein ID WP_001159871.1
Coordinates 93113..93418 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag C4E05_RS02170 Protein ID WP_000813634.1
Coordinates 93420..93638 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4E05_RS02135 88845..89870 + 1026 WP_000864945.1 zinc-binding alcohol dehydrogenase family protein -
C4E05_RS02140 89929..90600 - 672 WP_046788564.1 FadR family transcriptional regulator -
C4E05_RS02145 90695..91237 - 543 Protein_110 GGDEF domain-containing protein -
C4E05_RS02150 91254..91951 - 698 Protein_111 IS1 family transposase -
C4E05_RS02155 92003..92131 - 129 Protein_112 colicin M immunity protein -
C4E05_RS02160 92303..93112 - 810 WP_000016963.1 site-specific integrase -
C4E05_RS02165 93113..93418 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
C4E05_RS02170 93420..93638 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C4E05_RS02185 94106..94801 + 696 WP_072652234.1 DNA-binding protein -
C4E05_RS02190 94798..95418 + 621 WP_072652229.1 hypothetical protein -
C4E05_RS02195 95415..96203 + 789 WP_072652230.1 DUF2971 domain-containing protein -
C4E05_RS02200 96239..96979 + 741 WP_072652231.1 abortive infection family protein -
C4E05_RS02205 97135..97323 + 189 WP_000957857.1 hypothetical protein -
C4E05_RS02210 97333..98532 + 1200 WP_000948429.1 IS91 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / rmtB / sul1 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / tet(A) - 1..100229 100229
- flank IS/Tn - - 97333..98532 1199


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T95126 WP_001159871.1 NZ_CP026579:c93418-93113 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T95126 NZ_CP026579:c93418-93113 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT95126 WP_000813634.1 NZ_CP026579:c93638-93420 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT95126 NZ_CP026579:c93638-93420 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References