Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 44093..44694 Replicon plasmid pQnrS1_005237
Accession NZ_CP026578
Organism Escherichia coli strain WCHEC005237

Toxin (Protein)


Gene name doc Uniprot ID A0A5U0JPI4
Locus tag C4E05_RS01080 Protein ID WP_024166788.1
Coordinates 44093..44473 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A5T4YUW9
Locus tag C4E05_RS01085 Protein ID WP_033545779.1
Coordinates 44473..44694 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4E05_RS01060 40768..41710 + 943 Protein_22 IS3 family transposase -
C4E05_RS01065 41737..42433 - 697 Protein_23 IS1 family transposase -
C4E05_RS01070 42827..43855 + 1029 WP_072851570.1 tyrosine-type recombinase/integrase -
C4E05_RS01080 44093..44473 - 381 WP_024166788.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
C4E05_RS01085 44473..44694 - 222 WP_033545779.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
C4E05_RS01090 44767..45159 - 393 WP_000506718.1 hypothetical protein -
C4E05_RS01095 45271..45519 - 249 WP_001286530.1 DNA polymerase III subunit theta -
C4E05_RS01100 45522..45722 - 201 WP_001408980.1 hypothetical protein -
C4E05_RS01105 45858..46097 - 240 Protein_30 hypothetical protein -
C4E05_RS01110 46145..46285 - 141 WP_107524640.1 DUF4752 family protein -
C4E05_RS01115 46276..46980 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
C4E05_RS01120 47033..49246 + 2214 Protein_33 Tn3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / qnrS1 / dfrA14 / blaTEM-1B / floR - 1..121908 121908
- inside IScluster/Tn - - 40768..49246 8478


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(4-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13663.58 Da        Isoelectric Point: 5.6406

>T95121 WP_024166788.1 NZ_CP026578:c44473-44093 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVVYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELFGSNI

Download         Length: 381 bp

>T95121 NZ_CP026578:c44473-44093 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTGATAGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTCCAGGCCAGAGTTGTGTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGTATACAGGTATATGATTCTCCCGTGCTGGTGGAACTTGCGGTGGGGGCCGCAACTGG
TGAAATCCCCGTATCTTCAGTAGCGGAAAAACTACGTGAATTATTTGGTTCCAATATTTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8147.13 Da        Isoelectric Point: 4.7708

>AT95121 WP_033545779.1 NZ_CP026578:c44694-44473 [Escherichia coli]
MQSINFRTARGNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT95121 NZ_CP026578:c44694-44473 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTACTGAAGTGCTCAACAATGTTGAAGCTGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5U0JPI4

Antitoxin

Source ID Structure
AlphaFold DB A0A5T4YUW9

References