Detailed information of TA system TA095109

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 15261..15730
• Replicon: plasmid p5_tig9
• Accession: NZ_CP026567
• Organism: Pseudomonas avellanae strain R2leaf

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BKM03_RS31970
• Protein ID: WP_044322491.1
• Coordinates: 15449..15730 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q48BA1
• Locus tag: BKM03_RS31965
• Protein ID: WP_002556028.1
• Coordinates: 15261..15449 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BKM03_RS31935	10780..10957	-	178	Protein_8	integrase	-
BKM03_RS31940	11116..11766	+	651	WP_046036898.1	AAA family ATPase	-
BKM03_RS31945	11757..12116	+	360	WP_096103863.1	hypothetical protein	-
BKM03_RS31955	12843..14147	+	1305	WP_103377639.1	replication initiation protein	-
BKM03_RS31960	14448..15083	+	636	WP_031599320.1	recombinase family protein	-
BKM03_RS31965	15261..15449	+	189	WP_002556028.1	hypothetical protein	Antitoxin
BKM03_RS31970	15449..15730	+	282	WP_044322491.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BKM03_RS31975	16270..17328	+	1059	WP_103377640.1	avirulence protein	-
BKM03_RS31980	17611..19110	+	1500	WP_102136187.1	IS21-like element ISPsy20 family transposase	-
BKM03_RS31985	19103..19906	+	804	WP_104814063.1	IS21-like element ISPsy20 family helper ATPase IstB	-
BKM03_RS31990	20076..20231	+	156	Protein_18	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..20491	20491

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-85)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10676.29 Da
• Isoelectric Point: 6.7242

>T95109 WP_044322491.1 NZ_CP026567:15449-15730 [Pseudomonas avellanae]
MLPVVWLSPALDDLREIATYIAWENPSAARRLKSLLQEAIEPVAEHPYLYRSGRAPGTRELVAHPNYVLVYRVTLERIEV
VNVIHARQEYPSP

Nucleotide

• Length: 282 bp

>T95109 NZ_CP026567:15449-15730 [Pseudomonas avellanae]
ATGCTGCCGGTGGTCTGGCTCAGTCCCGCACTGGATGATCTGCGCGAAATCGCAACCTACATCGCATGGGAAAACCCGAG
CGCAGCGCGCCGGTTGAAAAGCCTCCTGCAGGAGGCTATCGAGCCGGTAGCCGAACACCCCTATTTATATCGTTCTGGGC
GAGCGCCAGGCACTCGCGAGCTGGTGGCTCACCCAAACTACGTCCTGGTCTATCGTGTCACCCTCGAGCGCATCGAGGTC
GTGAACGTAATCCACGCCAGGCAGGAATACCCCTCGCCCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 7043.86 Da
• Isoelectric Point: 4.2550

>AT95109 WP_002556028.1 NZ_CP026567:15261-15449 [Pseudomonas avellanae]
MANLSPIVSEFETDEQAASYDRWFRAKVAASLADPRPAIPHDEVMAEIEALIDQVETQREKR

Nucleotide

• Length: 189 bp

>AT95109 NZ_CP026567:15261-15449 [Pseudomonas avellanae]
ATGGCCAACCTCTCACCCATCGTTTCAGAATTCGAAACCGATGAACAGGCCGCAAGCTACGACCGCTGGTTTCGCGCCAA
GGTCGCCGCCAGTCTGGCCGATCCGCGTCCGGCCATCCCTCACGATGAGGTCATGGCGGAAATAGAAGCGCTGATCGATC
AGGTTGAAACCCAACGTGAGAAACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2G9KWB9