Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 9125..9798 Replicon plasmid p3_tig6
Accession NZ_CP026565
Organism Pseudomonas avellanae strain R2leaf

Toxin (Protein)


Gene name vapC Uniprot ID A0A2K4W2P1
Locus tag BKM03_RS31270 Protein ID WP_060402639.1
Coordinates 9379..9798 (+) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q48B52
Locus tag BKM03_RS31265 Protein ID WP_003381346.1
Coordinates 9125..9382 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BKM03_RS31235 5144..5749 - 606 WP_060403786.1 T3SS effector protein kinase HopBF1 -
BKM03_RS31240 5874..7106 + 1233 WP_104698134.1 IS91 family transposase -
BKM03_RS31250 7381..7662 + 282 WP_060402791.1 hypothetical protein -
BKM03_RS31255 7643..8872 + 1230 WP_065348575.1 IS91 family transposase -
BKM03_RS31265 9125..9382 + 258 WP_003381346.1 Arc family DNA-binding protein Antitoxin
BKM03_RS31270 9379..9798 + 420 WP_060402639.1 type II toxin-antitoxin system VapC family toxin Toxin
BKM03_RS31275 9839..10477 + 639 WP_060402640.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..69519 69519


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Arc-like DNA binding domain

(5-46)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 15164.46 Da        Isoelectric Point: 4.5482

>T95108 WP_060402639.1 NZ_CP026565:9379-9798 [Pseudomonas avellanae]
MILLDTNVISEPQRREPNAHVLDWIDAQALETLYLSTITVAELRAGIALMPVGKRQDSLRENLEKYLLPMFANRVLSFDM
TCTKAYAELLAKSRAAGLAVETADAFIAAIALANGFTVATRDTGPFEAAGLNVINPWEA

Download         Length: 420 bp

>T95108 NZ_CP026565:9379-9798 [Pseudomonas avellanae]
ATGATCCTGCTCGATACGAACGTAATTTCAGAACCGCAGCGCCGAGAGCCCAATGCCCACGTCCTTGACTGGATCGACGC
ACAAGCGCTGGAAACGCTTTATCTGTCCACCATCACGGTAGCCGAGTTGCGTGCAGGTATCGCGCTGATGCCTGTCGGAA
AACGCCAGGATAGCTTGCGTGAAAACCTGGAAAAGTACCTGCTGCCGATGTTCGCCAATAGGGTGCTGTCGTTCGATATG
ACTTGTACGAAGGCTTATGCCGAACTGCTGGCCAAGTCCCGCGCAGCCGGGTTGGCGGTCGAAACGGCAGATGCCTTCAT
TGCAGCCATCGCCCTCGCCAACGGCTTCACCGTCGCCACCCGTGACACTGGCCCTTTTGAGGCTGCCGGGTTGAATGTCA
TTAACCCCTGGGAAGCGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9401.62 Da        Isoelectric Point: 9.0330

>AT95108 WP_003381346.1 NZ_CP026565:9125-9382 [Pseudomonas avellanae]
VANVNVRNLPDEVHRAIRIQAALHGRSTEAEIRDILERAARPEGRVKLGSFLASIAREVGGLTDKEHTLFENTRITSPAR
AVSFE

Download         Length: 258 bp

>AT95108 NZ_CP026565:9125-9382 [Pseudomonas avellanae]
GTGGCAAACGTGAACGTAAGAAATCTACCCGATGAAGTGCATCGGGCAATCCGCATCCAAGCTGCCCTGCACGGGCGCAG
CACCGAGGCCGAAATACGCGACATCTTGGAGCGGGCGGCGAGACCCGAGGGACGTGTGAAACTTGGCTCGTTCTTGGCGT
CTATTGCCCGTGAGGTTGGTGGTCTGACCGATAAAGAACACACCTTGTTTGAGAACACGCGCATCACGTCTCCGGCCCGT
GCGGTGAGCTTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2K4W2P1

Antitoxin

Source ID Structure
AlphaFold DB Q48B52

References