Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 21372..21919 Replicon plasmid p2_tig5
Accession NZ_CP026564
Organism Pseudomonas avellanae strain R2leaf

Toxin (Protein)


Gene name mazF Uniprot ID Q48B48
Locus tag BKM03_RS30650 Protein ID WP_003319696.1
Coordinates 21372..21698 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID H1ZWZ3
Locus tag BKM03_RS30655 Protein ID WP_015060676.1
Coordinates 21695..21919 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BKM03_RS30620 16383..16892 - 510 WP_005735414.1 peptide deformylase -
BKM03_RS30630 17180..18409 - 1230 WP_104698143.1 IS91 family transposase -
BKM03_RS30640 19143..20123 + 981 WP_060402216.1 IS5 family transposase -
BKM03_RS30645 20247..21164 + 918 Protein_24 tyrosine-protein phosphatase -
BKM03_RS30650 21372..21698 - 327 WP_003319696.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BKM03_RS30655 21695..21919 - 225 WP_015060676.1 antitoxin MazE family protein Antitoxin
BKM03_RS30660 22165..22800 + 636 WP_060402215.1 recombinase family protein -
BKM03_RS30670 23972..24340 + 369 WP_003348806.1 hypothetical protein -
BKM03_RS30675 24402..25064 - 663 Protein_29 Tn3 family transposase -
BKM03_RS30680 25136..25789 - 654 WP_044311043.1 type III effector HopAZ1 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..102862 102862
- inside IScluster/Tn - - 769..41216 40447


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-103)

Antitoxin

Antitoxin MazE-like

(8-72)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11660.71 Da        Isoelectric Point: 8.0546

>T95107 WP_003319696.1 NZ_CP026564:c21698-21372 [Pseudomonas avellanae]
MMRGDLVTVAMQGDFGKPRPALVIQANQFSEHTSVTVLPVTSTLVAAPLLRITVQPTAENGLQKPSQVMLDKTMTLKREK
IGPTFGHIGVDSMVEVERCLAVFLGIAN

Download         Length: 327 bp

>T95107 NZ_CP026564:c21698-21372 [Pseudomonas avellanae]
ATGATGCGAGGTGACCTAGTCACCGTCGCCATGCAAGGTGACTTTGGTAAACCAAGACCCGCCTTGGTAATTCAGGCAAA
CCAATTCAGCGAACACACCAGCGTTACCGTGCTGCCAGTGACCAGCACCCTAGTCGCAGCACCGCTGCTGCGCATCACCG
TTCAGCCGACCGCAGAAAACGGCTTGCAGAAGCCTTCTCAGGTCATGCTAGACAAGACCATGACACTCAAGCGTGAAAAA
ATAGGCCCGACTTTTGGGCATATCGGAGTGGATTCAATGGTGGAGGTGGAGCGCTGTTTAGCCGTGTTCTTGGGTATCGC
CAATTGA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8515.62 Da        Isoelectric Point: 5.5294

>AT95107 WP_015060676.1 NZ_CP026564:c21919-21695 [Pseudomonas avellanae]
MPTSHVNDRVQRHRDALRAAGLRPIQIWVPDTRRPDFAQECHRQCLLVAQADMANTYMQGLMDEALDDVEGWTP

Download         Length: 225 bp

>AT95107 NZ_CP026564:c21919-21695 [Pseudomonas avellanae]
ATGCCTACATCCCATGTAAATGACCGCGTTCAACGCCATCGCGACGCGCTGCGTGCGGCTGGCCTACGGCCCATTCAGAT
TTGGGTTCCCGATACCCGGCGGCCAGACTTTGCACAGGAGTGCCATCGACAGTGCCTGTTGGTTGCTCAGGCAGACATGG
CCAATACCTATATGCAGGGGTTGATGGACGAAGCCTTAGACGATGTTGAGGGTTGGACGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V8S2J7

Antitoxin

Source ID Structure
AlphaFold DB A0A330JWK7

References