Detailed information of TA system TA095105

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 4281977..4282588
• Replicon: chromosome
• Accession: NZ_CP026562
• Organism: Pseudomonas avellanae strain R2leaf

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: A0A2S4HLU6
• Locus tag: BKM03_RS20395
• Protein ID: WP_005735311.1
• Coordinates: 4282238..4282588 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A3M4KYK1
• Locus tag: BKM03_RS20390
• Protein ID: WP_005735310.1
• Coordinates: 4281977..4282225 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BKM03_RS20355	4277137..4278204	+	1068	WP_032685503.1	IS630-like element ISPsy32 family transposase	-
BKM03_RS20360	4278230..4278598	+	369	Protein_3748	DUF2778 domain-containing protein	-
BKM03_RS20365	4278598..4278927	+	330	WP_005735474.1	hypothetical protein	-
BKM03_RS20370	4279111..4279602	+	492	Protein_3750	IS5 family transposase	-
BKM03_RS20375	4279682..4280704	+	1023	WP_002551322.1	IS21-like element ISPsy4 family transposase	-
BKM03_RS20380	4280701..4281510	+	810	WP_005741073.1	IS21-like element ISPsy4 family helper ATPase IstB	-
BKM03_RS20385	4281559..4281816	+	258	Protein_3753	transposase	-
BKM03_RS20390	4281977..4282225	+	249	WP_005735310.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BKM03_RS20395	4282238..4282588	+	351	WP_005735311.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BKM03_RS20400	4282694..4283101	-	408	WP_103377658.1	hypothetical protein	-
BKM03_RS20410	4283393..4283752	+	360	Protein_3757	helix-turn-helix domain containing protein	-
BKM03_RS20415	4283840..4284196	+	357	WP_078983211.1	IS66 family insertion sequence element accessory protein TnpB	-
BKM03_RS20420	4284214..4285746	+	1533	WP_104698608.1	IS66 family transposase	-
BKM03_RS20425	4285792..4286508	+	717	Protein_3760	IS630 family transposase	-
BKM03_RS32715	4287202..4287441	+	240	WP_005740586.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	4277473..4286508	9035

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12901.70 Da
• Isoelectric Point: 4.1824

>T95105 WP_005735311.1 NZ_CP026562:4282238-4282588 [Pseudomonas avellanae]
MNTFALRFTDVAQQSLEDQVEHLAVTQGFSSAAQRIDTLIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMDSDGITDIAVLLVLGGKQSVEQALIRYCLLQPI

Nucleotide

• Length: 351 bp

>T95105 NZ_CP026562:4282238-4282588 [Pseudomonas avellanae]
ATGAATACGTTTGCACTGCGCTTTACTGATGTTGCTCAGCAAAGCCTCGAAGATCAGGTTGAACACCTGGCTGTCACTCA
GGGATTCTCATCAGCTGCTCAACGCATAGATACCCTTATCGATGCCATCCAGGACAAGCTCCTGTCCACCCCGTTGGGCT
ACCCGGTCAGTCCTCAATTGAGTGAGCTGGGTGTACTGCACTATCGTGAGTTGAACACTGACGGATACCGGATCTTTTAC
GAGGTCATGGATTCTGATGGTATTACCGACATCGCCGTATTGCTCGTATTAGGCGGGAAACAAAGTGTTGAACAAGCCCT
GATCCGCTATTGCCTTCTTCAGCCTATATAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9359.74 Da
• Isoelectric Point: 9.8452

>AT95105 WP_005735310.1 NZ_CP026562:4281977-4282225 [Pseudomonas avellanae]
MRVETISYLKRNAADLPLDEPLIVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYAQGKHCSTDELKARLSRRFA
HK

Nucleotide

• Length: 249 bp

>AT95105 NZ_CP026562:4281977-4282225 [Pseudomonas avellanae]
ATGCGAGTTGAAACAATAAGTTATCTGAAACGCAACGCAGCCGACCTGCCTCTCGATGAGCCACTGATTGTCACGCAAAA
CGGTGTCCCGGCCTATGTGGTGGAGTCTTACGCAGATCGTAAGCGCCGCGATGAGTCGATCGCCTTGGTGAAGCTCCTGG
CAATCAGTTCCCGCGAGTACGCCCAAGGCAAGCACTGTTCCACTGACGAACTCAAAGCCCGATTGAGCAGGAGGTTTGCC
CACAAATAA

Structures

Toxin

• AlphaFold DB: A0A2S4HLU6

Antitoxin

• AlphaFold DB: A0A3M4KYK1