Detailed information of TA system TA095103

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 587980..589019
• Replicon: chromosome
• Accession: NZ_CP026562
• Organism: Pseudomonas avellanae strain R2leaf

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: BKM03_RS02960
• Protein ID: WP_005737551.1
• Coordinates: 588444..589019 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A2G9KX16
• Locus tag: BKM03_RS02955
• Protein ID: WP_005737550.1
• Coordinates: 587980..588447 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BKM03_RS02940	585858..586475	+	618	WP_005737548.1	4'-phosphopantetheinyl transferase superfamily protein	-
BKM03_RS02945	586570..587163	-	594	WP_005737549.1	hypothetical protein	-
BKM03_RS32505	587220..587705	-	486	Protein_538	hypothetical protein	-
BKM03_RS02955	587980..588447	+	468	WP_005737550.1	helix-turn-helix domain-containing protein	Antitoxin
BKM03_RS02960	588444..589019	+	576	WP_005737551.1	PIN domain-containing protein	Toxin
BKM03_RS02965	589200..589451	+	252	WP_005737552.1	helix-turn-helix domain-containing protein	-
BKM03_RS02970	589451..590647	+	1197	WP_005737554.1	type II toxin-antitoxin system HipA family toxin	-
BKM03_RS02975	590768..591052	+	285	Protein_543	N-acetylmuramoyl-L-alanine amidase	-
BKM03_RS02980	591068..591817	-	750	WP_103377603.1	IS5 family transposase	-
BKM03_RS02990	592319..592561	+	243	Protein_545	IS21-like element ISPsy4 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21944.11 Da
• Isoelectric Point: 5.5934

>T95103 WP_005737551.1 NZ_CP026562:588444-589019 [Pseudomonas avellanae]
MRHSPFTAFYDANVLYPAPLRDFLMHLALTGVYRARWSSQIHDEWKRNLLINRPELTREQLDRTSALMDKAVPDGLVSDY
QSLIEGLKLPDADDRHVLAAAIKCNASVIVTFNLKDFPKDVLDTFDMEPLHPDDFIADLWDLDKAAVLEAAQRQRASLRN
PAHSARQYLDKLLQQKLPETVKLLSDFEFLI

Nucleotide

• Length: 576 bp

>T95103 NZ_CP026562:588444-589019 [Pseudomonas avellanae]
ATGAGGCATTCGCCGTTTACAGCTTTCTATGACGCCAACGTCCTATACCCGGCACCGCTTCGTGACTTTCTGATGCACTT
GGCATTGACTGGCGTGTACCGCGCACGCTGGTCTTCTCAAATTCATGACGAGTGGAAGCGCAACCTTCTGATCAATCGTC
CAGAGCTTACCCGTGAGCAACTGGATCGTACGTCTGCGCTGATGGATAAGGCAGTGCCGGACGGTCTGGTATCTGATTAC
CAGTCGCTAATTGAAGGGCTTAAGCTGCCTGACGCCGATGATCGTCATGTGCTGGCGGCGGCAATCAAGTGCAATGCCTC
TGTCATCGTGACGTTCAACCTCAAGGACTTTCCGAAAGACGTCCTCGATACGTTCGATATGGAGCCCTTGCACCCGGATG
ATTTCATTGCCGACCTTTGGGATCTCGACAAAGCCGCTGTTCTCGAAGCCGCACAACGGCAGCGAGCATCTTTGAGAAAC
CCAGCACACAGTGCAAGGCAATACCTCGATAAGCTGTTGCAGCAGAAGCTTCCCGAGACTGTAAAACTGCTTTCGGATTT
TGAGTTTCTGATCTGA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17233.75 Da
• Isoelectric Point: 5.2911

>AT95103 WP_005737550.1 NZ_CP026562:587980-588447 [Pseudomonas avellanae]
MSSADATRTVLPGEKEIAAAVESSRQLAAFLTTKCDTQRIELVDETQQREVVELPMFALRLLGEILSELALGNAVKVVPI
HAELTTQEAADMLNVSRPHLVKMLDQGMLPHTKTGRHRRVKFADLMSYKQQRDQASREAMDELAAQAQELGMGYE

Nucleotide

• Length: 468 bp

>AT95103 NZ_CP026562:587980-588447 [Pseudomonas avellanae]
ATGAGCAGCGCAGACGCCACTCGCACCGTTCTTCCTGGGGAGAAAGAAATCGCTGCGGCGGTTGAATCAAGTCGGCAATT
AGCCGCGTTTCTTACAACGAAATGTGACACCCAGCGCATTGAGCTTGTGGACGAAACGCAACAGCGCGAAGTCGTCGAGC
TGCCTATGTTTGCACTCCGTTTGCTCGGCGAAATTCTCAGTGAGCTTGCGCTGGGTAACGCCGTCAAGGTGGTCCCCATT
CATGCTGAACTCACTACTCAGGAAGCAGCAGACATGCTCAATGTGTCCCGGCCTCATCTGGTGAAGATGCTGGACCAAGG
CATGTTGCCGCACACCAAAACCGGCAGGCACCGCCGAGTCAAATTTGCTGATTTGATGAGCTACAAGCAGCAGCGCGACC
AGGCAAGTCGCGAAGCCATGGACGAGTTGGCCGCGCAAGCCCAAGAACTAGGGATGGGATATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2G9KX16