Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 85404..86040 Replicon plasmid p2_tig3
Accession NZ_CP026559
Organism Pseudomonas amygdali pv. morsprunorum strain R15244

Toxin (Protein)


Gene name hicA Uniprot ID A0A2S4IFM0
Locus tag BKM19_RS30420 Protein ID WP_044310977.1
Coordinates 85404..85586 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A2S4IFN3
Locus tag BKM19_RS30425 Protein ID WP_060404204.1
Coordinates 85618..86040 (+) Length 141 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BKM19_RS30395 81858..83087 - 1230 WP_104721077.1 IS91 family transposase -
BKM19_RS30400 83068..83349 - 282 WP_060407302.1 hypothetical protein -
BKM19_RS30410 83623..84849 - 1227 WP_104721076.1 IS91 family transposase -
BKM19_RS30415 84830..85111 - 282 WP_060402791.1 hypothetical protein -
BKM19_RS30420 85404..85586 + 183 WP_044310977.1 type II toxin-antitoxin system HicA family toxin Toxin
BKM19_RS30425 85618..86040 + 423 WP_060404204.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BKM19_RS30430 86206..86517 + 312 Protein_109 hypothetical protein -
BKM19_RS30435 86549..89575 - 3027 WP_060404205.1 Tn3-like element ISPsy30 family transposase -
BKM19_RS30440 89565..90161 - 597 WP_057425230.1 recombinase family protein -
BKM19_RS32335 90478..91008 - 531 WP_005752338.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..168854 168854
- inside IScluster/Tn - - 77838..99433 21595


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-58)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6836.96 Da        Isoelectric Point: 10.5498

>T95101 WP_044310977.1 NZ_CP026559:85404-85586 [Pseudomonas amygdali pv. morsprunorum]
MKCSEFRRWLQAQGAEFKAAKGSHFKVYLNGKATIFADHGSKEMHEGLRKTIIKQLGLKD

Download         Length: 183 bp

>T95101 NZ_CP026559:85404-85586 [Pseudomonas amygdali pv. morsprunorum]
ATGAAGTGCAGCGAGTTTCGGCGATGGTTACAGGCTCAAGGCGCTGAGTTCAAAGCGGCCAAGGGCAGTCACTTCAAGGT
GTACTTGAATGGCAAGGCGACCATCTTTGCAGACCACGGCAGCAAGGAAATGCACGAAGGGTTACGCAAAACGATCATCA
AACAGCTGGGCCTCAAGGACTGA

Antitoxin


Download         Length: 141 a.a.        Molecular weight: 15616.00 Da        Isoelectric Point: 5.1895

>AT95101 WP_060404204.1 NZ_CP026559:85618-86040 [Pseudomonas amygdali pv. morsprunorum]
MYQYPLTQHQEQTGVWLSCPNIPEMNASGETLTEALDEALNGMESALSLYVDQRRKIPQASLPVGDELVMHLPALTVAKI
MLWNSMLDNGVSRAELARRLGCTRQVVDRLVDFLHTSKIEQVERALGLLGRRITLSLEAA

Download         Length: 423 bp

>AT95101 NZ_CP026559:85618-86040 [Pseudomonas amygdali pv. morsprunorum]
ATGTATCAGTATCCGCTGACGCAGCATCAAGAACAGACTGGCGTCTGGCTTTCTTGCCCGAACATCCCCGAAATGAACGC
ATCAGGTGAAACCTTGACCGAGGCCCTGGACGAAGCCCTGAACGGTATGGAGTCGGCATTGTCGCTCTATGTCGACCAGC
GCCGAAAAATCCCGCAGGCGTCACTACCAGTCGGTGACGAGCTGGTGATGCACCTGCCTGCGCTGACCGTCGCCAAGATC
ATGCTGTGGAACAGCATGTTGGACAACGGCGTGAGCCGTGCGGAGCTGGCGCGCCGCCTGGGCTGTACGCGGCAAGTCGT
GGATCGGCTGGTGGACTTCCTGCACACCTCGAAAATCGAGCAGGTCGAGCGAGCGCTAGGCCTACTGGGCAGGCGTATCA
CGCTGAGCCTTGAGGCGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S4IFM0

Antitoxin

Source ID Structure
AlphaFold DB A0A2S4IFN3

References