Detailed information of TA system TA095096

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 192263..192874
• Replicon: chromosome
• Accession: NZ_CP026558
• Organism: Pseudomonas amygdali pv. morsprunorum strain R15244

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: A0A2G4CXS4
• Locus tag: BKM19_RS01590
• Protein ID: WP_057456423.1
• Coordinates: 192524..192874 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: -
• Locus tag: BKM19_RS01585
• Protein ID: WP_057432311.1
• Coordinates: 192263..192511 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BKM19_RS01550	187552..187752	+	201	WP_055001606.1	hypothetical protein	-
BKM19_RS01555	187819..188097	-	279	WP_004661031.1	mobilization protein	-
BKM19_RS01565	188395..188745	-	351	WP_060407318.1	hypothetical protein	-
BKM19_RS01570	189005..189334	+	330	WP_003344297.1	ribbon-helix-helix protein, CopG family	-
BKM19_RS01575	189345..191300	+	1956	WP_007247497.1	relaxase/mobilization nuclease domain-containing protein	-
BKM19_RS01580	191325..192008	-	684	WP_032640233.1	hypothetical protein	-
BKM19_RS01585	192263..192511	+	249	WP_057432311.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BKM19_RS01590	192524..192874	+	351	WP_057456423.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BKM19_RS01595	192981..193385	-	405	WP_057456424.1	hypothetical protein	-
BKM19_RS01600	193375..196197	-	2823	WP_060403779.1	RHS repeat protein	-
BKM19_RS01605	196194..196985	-	792	WP_057431753.1	RHS repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	113913..273504	159591

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12901.70 Da
• Isoelectric Point: 4.1824

>T95096 WP_057456423.1 NZ_CP026558:192524-192874 [Pseudomonas amygdali pv. morsprunorum]
MNTFALRFTDLAQQSLEDQVEHLAVTQGFSSAAQRIDSLIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMDSDGITDIAVLLVLGGKQSVEQALIRYCLLQPI

Nucleotide

• Length: 351 bp

>T95096 NZ_CP026558:192524-192874 [Pseudomonas amygdali pv. morsprunorum]
ATGAATACGTTTGCACTGCGCTTTACCGATCTTGCCCAGCAAAGCCTTGAAGACCAGGTTGAACACTTGGCTGTCACTCA
GGGATTCTCATCAGCTGCTCAACGCATAGATTCCCTTATCGATGCCATCCAGGACAAGCTCCTGTCCACCCCGTTGGGCT
ACCCGGTCAGTCCACAGTTGAGTGAGCTGGGTGTACTGCACTATCGTGAGTTGAACACTGACGGATACCGGATCTTTTAT
GAGGTGATGGATTCTGATGGTATTACCGATATCGCCGTATTGCTCGTATTAGGCGGGAAACAAAGTGTTGAACAAGCCCT
CATCCGCTATTGCCTTCTTCAGCCTATATAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9343.74 Da
• Isoelectric Point: 9.8452

>AT95096 WP_057432311.1 NZ_CP026558:192263-192511 [Pseudomonas amygdali pv. morsprunorum]
MRVETISYLKRNAADLPLDEPLIVTQNGVPAYVVESYADRKRRDEAIALVKLLAISSREYAQGKHCSTDELKARLSRRFA
HK

Nucleotide

• Length: 249 bp

>AT95096 NZ_CP026558:192263-192511 [Pseudomonas amygdali pv. morsprunorum]
ATGCGAGTTGAAACAATAAGCTACCTGAAACGCAATGCAGCCGACCTGCCTCTCGATGAGCCATTGATCGTCACGCAAAA
CGGTGTCCCGGCCTACGTGGTGGAGTCTTACGCGGATCGTAAGCGCCGCGATGAGGCCATTGCCTTGGTAAAGCTCTTGG
CCATCAGTTCCCGCGAGTACGCCCAAGGCAAGCACTGTTCCACTGACGAACTCAAAGCCCGATTGAGCAGGAGGTTTGCC
CACAAGTAA

Structures

Toxin

• AlphaFold DB: A0A2G4CXS4