Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/-
Location 156542..157116 Replicon chromosome
Accession NZ_CP026558
Organism Pseudomonas amygdali pv. morsprunorum strain R15244

Toxin (Protein)


Gene name vapC Uniprot ID Q48BC5
Locus tag BKM19_RS01320 Protein ID WP_003344395.1
Coordinates 156542..156919 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q48BC4
Locus tag BKM19_RS01325 Protein ID WP_004644067.1
Coordinates 156916..157116 (-) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BKM19_RS01300 151758..152336 + 579 WP_004642775.1 conjugal transfer protein TraX -
BKM19_RS01305 152467..154611 + 2145 WP_060404099.1 DotA/TraY family protein -
BKM19_RS01310 154598..155257 + 660 WP_044424882.1 plasmid IncI1-type surface exclusion protein ExcA -
BKM19_RS01315 155350..156552 + 1203 WP_044424879.1 hypothetical protein -
BKM19_RS01320 156542..156919 - 378 WP_003344395.1 PIN domain-containing protein Toxin
BKM19_RS01325 156916..157116 - 201 WP_004644067.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
BKM19_RS01330 157209..157382 + 174 Protein_147 conjugal transfer protein TrbB -
BKM19_RS01340 157565..158794 - 1230 WP_104808010.1 IS91 family transposase -
BKM19_RS01345 158775..159056 - 282 WP_060402791.1 hypothetical protein -
BKM19_RS01350 159098..159493 + 396 WP_060403043.1 hypothetical protein -
BKM19_RS01355 159478..160446 + 969 WP_044422905.1 AAA family ATPase -
BKM19_RS01360 160586..161878 - 1293 WP_060403044.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 113913..273504 159591
- flank IS/Tn - - 157565..158773 1208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-113)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-43)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13633.73 Da        Isoelectric Point: 6.4723

>T95094 WP_003344395.1 NZ_CP026558:c156919-156542 [Pseudomonas amygdali pv. morsprunorum]
VKGVLVDTSVWVEHFRNNSPELVNLLSQDRVLIHPMVIGELACGTPPDRSNTLTDLGDLRGAQQPTVSEVIAFLNTHKLY
GLGCGLVDMTLLASALLSGTALWTLDKRLERLASRMAVSYQPPTH

Download         Length: 378 bp

>T95094 NZ_CP026558:c156919-156542 [Pseudomonas amygdali pv. morsprunorum]
GTGAAAGGCGTCCTGGTAGACACCTCCGTTTGGGTTGAGCACTTTCGCAACAACAGTCCAGAACTGGTGAACTTGCTCAG
CCAGGACCGGGTACTCATACACCCCATGGTGATCGGAGAACTGGCGTGTGGTACGCCACCTGATCGTTCGAACACCTTGA
CCGACCTTGGCGACCTACGAGGTGCACAACAACCCACGGTGAGTGAAGTCATCGCCTTTCTGAACACCCACAAGCTCTAT
GGCCTGGGCTGTGGGCTGGTGGATATGACGCTCCTGGCCTCCGCGTTATTGAGCGGCACAGCGCTTTGGACGTTAGACAA
GCGCCTGGAGCGTTTGGCTAGTCGCATGGCCGTGTCTTACCAGCCTCCTACTCACTAG

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7445.66 Da        Isoelectric Point: 7.0102

>AT95094 WP_004644067.1 NZ_CP026558:c157116-156916 [Pseudomonas amygdali pv. morsprunorum]
MRTTVTIDDALYQRALEVADPAMDKADLFREAVQTFVRIQAAKRLMALGATLPTMEDIARRHEKAL

Download         Length: 201 bp

>AT95094 NZ_CP026558:c157116-156916 [Pseudomonas amygdali pv. morsprunorum]
ATGAGAACGACCGTCACCATCGACGATGCGTTGTACCAACGTGCTCTGGAAGTCGCCGATCCGGCGATGGATAAGGCCGA
CCTGTTCCGTGAAGCCGTTCAGACGTTTGTGCGCATTCAAGCAGCTAAACGATTGATGGCCTTAGGGGCCACACTGCCAA
CGATGGAAGACATCGCCCGCCGACACGAGAAGGCACTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3GPE2

Antitoxin

Source ID Structure
AlphaFold DB A0A2S4IKB9

References