Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 49142..49689 Replicon plasmid p1_tig4
Accession NZ_CP026557
Organism Pseudomonas amygdali pv. morsprunorum strain R15244

Toxin (Protein)


Gene name mazF Uniprot ID Q48B48
Locus tag BKM19_RS00255 Protein ID WP_003319696.1
Coordinates 49142..49468 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7V8M172
Locus tag BKM19_RS00260 Protein ID WP_003319697.1
Coordinates 49465..49689 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BKM19_RS00225 44265..44462 + 198 WP_010209245.1 ribbon-helix-helix protein, CopG family -
BKM19_RS00230 44810..46123 + 1314 WP_032489454.1 replication initiation protein -
BKM19_RS00235 46262..46687 + 426 WP_010209623.1 S24 family peptidase -
BKM19_RS00240 46665..47963 + 1299 WP_032642043.1 Y-family DNA polymerase -
BKM19_RS00245 48180..48560 - 381 Protein_47 hypothetical protein -
BKM19_RS00250 48789..49070 - 282 Protein_48 IS5/IS1182 family transposase -
BKM19_RS00255 49142..49468 - 327 WP_003319696.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BKM19_RS00260 49465..49689 - 225 WP_003319697.1 antitoxin MazE family protein Antitoxin
BKM19_RS00265 49962..50594 - 633 WP_024421175.1 recombinase family protein -
BKM19_RS00270 50898..51845 - 948 WP_005769731.1 hypothetical protein -
BKM19_RS00275 52207..52488 - 282 WP_004666803.1 hypothetical protein -
BKM19_RS00280 52488..53171 - 684 WP_002556051.1 AAA family ATPase -
BKM19_RS00285 53566..53766 + 201 WP_002556050.1 molecular chaperone DnaK -
BKM19_RS00290 53804..54025 + 222 WP_004666801.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..81536 81536


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-103)

Antitoxin

Antitoxin MazE-like

(8-72)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11660.71 Da        Isoelectric Point: 8.0546

>T95093 WP_003319696.1 NZ_CP026557:c49468-49142 [Pseudomonas amygdali pv. morsprunorum]
MMRGDLVTVAMQGDFGKPRPALVIQANQFSEHTSVTVLPVTSTLVAAPLLRITVQPTAENGLQKPSQVMLDKTMTLKREK
IGPTFGHIGVDSMVEVERCLAVFLGIAN

Download         Length: 327 bp

>T95093 NZ_CP026557:c49468-49142 [Pseudomonas amygdali pv. morsprunorum]
ATGATGCGAGGTGACCTAGTCACCGTCGCCATGCAAGGTGACTTTGGTAAACCAAGACCCGCCTTGGTAATTCAGGCAAA
CCAATTCAGCGAACACACCAGCGTTACCGTGCTGCCAGTGACCAGCACCCTAGTCGCAGCACCGCTGCTGCGCATCACCG
TTCAGCCGACCGCAGAAAACGGCTTGCAGAAGCCTTCTCAGGTCATGCTAGACAAGACCATGACCCTCAAGCGTGAAAAA
ATAGGCCCGACTTTTGGGCATATCGGAGTGGATTCAATGGTGGAGGTAGAGCGCTGTTTAGCCGTGTTCTTGGGTATCGC
CAATTGA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8471.61 Da        Isoelectric Point: 6.2008

>AT95093 WP_003319697.1 NZ_CP026557:c49689-49465 [Pseudomonas amygdali pv. morsprunorum]
MPTSHVNARVQRHRDALRAAGLRPIQIWVPDTRRPDFAQECHRQCLLVAQADMANTYMQGLMDEALDDVEGWTP

Download         Length: 225 bp

>AT95093 NZ_CP026557:c49689-49465 [Pseudomonas amygdali pv. morsprunorum]
ATGCCTACATCCCATGTAAATGCCCGCGTTCAACGCCATCGCGACGCGCTGCGTGCGGCTGGCCTACGGCCCATTCAGAT
TTGGGTTCCCGATACCCGGCGGCCAGACTTTGCACAGGAGTGCCATCGACAGTGCCTGTTGGTTGCTCAGGCAGACATGG
CCAATACCTATATGCAGGGGTTGATGGACGAAGCCTTAGACGATGTTGAGGGTTGGACGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V8S2J7

Antitoxin

Source ID Structure
AlphaFold DB A0A7V8M172

References