Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazE-MazF
Location 97308..97870 Replicon plasmid unnamed3
Accession NZ_CP026547
Organism Cupriavidus metallidurans strain Ni-2

Toxin (Protein)


Gene name mazF Uniprot ID A0A132H7S3
Locus tag C3Z06_RS32345 Protein ID WP_024569645.1
Coordinates 97308..97556 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A132H6K9
Locus tag C3Z06_RS32350 Protein ID WP_024569644.1
Coordinates 97550..97870 (+) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3Z06_RS33290 93707..94252 - 546 WP_124682078.1 transposase -
C3Z06_RS32330 94750..95241 + 492 WP_158664445.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
C3Z06_RS32335 95251..96288 + 1038 WP_024569647.1 GIY-YIG nuclease family protein -
C3Z06_RS32340 96285..96812 + 528 WP_024569646.1 hypothetical protein -
C3Z06_RS32345 97308..97556 + 249 WP_024569645.1 antitoxin Toxin
C3Z06_RS32350 97550..97870 + 321 WP_024569644.1 type II toxin-antitoxin system PemK/MazF family toxin Antitoxin
C3Z06_RS32355 97946..99132 + 1187 WP_085960481.1 IS3-like element ISRme13 family transposase -
C3Z06_RS32365 99590..99829 + 240 WP_024569642.1 hypothetical protein -
C3Z06_RS32370 99842..100159 + 318 WP_024569641.1 hypothetical protein -
C3Z06_RS32375 100294..100605 + 312 WP_024569640.1 hypothetical protein -
C3Z06_RS33295 100626..100991 + 366 WP_035835006.1 hypothetical protein -
C3Z06_RS32380 101238..101444 + 207 WP_024569639.1 hypothetical protein -
C3Z06_RS32385 101566..102003 + 438 WP_024569638.1 hypothetical protein -
C3Z06_RS33300 102167..102448 + 282 WP_152536340.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..180778 180778


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

mRNA interferase PemK-like

(4-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 8910.16 Da        Isoelectric Point: 4.2463

>T95090 WP_024569645.1 NZ_CP026547:97308-97556 [Cupriavidus metallidurans]
MRVTIRRMGNSQGVLIPKPILAQLGLEDEVDMAVEDDTLVIRKPKKKAREGWAEASEAVAAAGDDALVMGEFSNADDAEL
VW

Download         Length: 249 bp

>T95090 NZ_CP026547:97308-97556 [Cupriavidus metallidurans]
ATGAGAGTAACGATTCGCAGAATGGGCAACTCGCAGGGGGTGCTAATCCCCAAACCGATCCTTGCCCAGCTTGGTTTGGA
GGATGAAGTGGACATGGCAGTTGAGGACGACACGCTGGTCATTCGCAAGCCTAAGAAGAAGGCGCGCGAGGGCTGGGCAG
AAGCCAGCGAAGCTGTCGCAGCGGCTGGCGACGACGCGCTGGTAATGGGCGAGTTTTCCAATGCCGATGACGCGGAGCTG
GTGTGGTAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11725.96 Da        Isoelectric Point: 10.7695

>AT95090 WP_024569644.1 NZ_CP026547:97550-97870 [Cupriavidus metallidurans]
VVARGEIWLVALDPTKGSEIQKTRPCVVISPPEMHDHLRTVIVAPMTTKGRAAPFRVPITFMRKNGLILLDQLRAVDKVR
LIKKAGAVADKTLLSTLQTLQEVFAE

Download         Length: 321 bp

>AT95090 NZ_CP026547:97550-97870 [Cupriavidus metallidurans]
GTGGTAGCGCGCGGAGAGATTTGGCTGGTCGCTCTGGACCCGACAAAGGGCAGTGAGATTCAGAAGACGCGACCGTGCGT
GGTCATCTCGCCTCCGGAAATGCACGACCATTTGCGCACGGTGATCGTTGCGCCGATGACGACAAAGGGCCGGGCGGCAC
CGTTTCGTGTGCCTATCACCTTCATGCGCAAGAATGGACTGATTTTGCTAGATCAGCTTCGTGCAGTAGACAAGGTGCGC
TTGATCAAGAAGGCCGGCGCCGTTGCCGATAAGACGTTGTTGAGCACCCTGCAAACATTGCAGGAAGTCTTTGCAGAGTA
G

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A132H7S3

Antitoxin

Source ID Structure
AlphaFold DB A0A132H6K9

References