Detailed information of TA system TA095089

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 5191079..5192121
• Replicon: chromosome
• Accession: NZ_CP026544
• Organism: Cupriavidus metallidurans strain Ni-2

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: C3Z06_RS24335
• Protein ID: WP_003109777.1
• Coordinates: 5191079..5191654 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: C3Z06_RS24340
• Protein ID: WP_003050245.1
• Coordinates: 5191651..5192121 (-)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3Z06_RS24310	5187517..5188242	-	726	WP_003050273.1	CBASS effector endonuclease NucC	-
C3Z06_RS24315	5188281..5189183	-	903	WP_003105624.1	nucleotidyltransferase domain-containing protein	-
C3Z06_RS24320	5189183..5189683	-	501	WP_003090159.1	hypothetical protein	-
C3Z06_RS24325	5189680..5190150	-	471	WP_003105626.1	hypothetical protein	-
C3Z06_RS24330	5190147..5191061	-	915	WP_042178573.1	AAA family ATPase	-
C3Z06_RS24335	5191079..5191654	-	576	WP_003109777.1	PIN domain-containing protein	Toxin
C3Z06_RS24340	5191651..5192121	-	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
C3Z06_RS24345	5192325..5192708	+	384	WP_003120001.1	RAQPRD family integrative conjugative element protein	-
C3Z06_RS24350	5192705..5192938	+	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
C3Z06_RS24355	5192955..5193314	+	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
C3Z06_RS24360	5193326..5193724	+	399	WP_003105639.1	TIGR03750 family conjugal transfer protein	-
C3Z06_RS24365	5193721..5194413	+	693	WP_003105641.1	TIGR03746 family integrating conjugative element protein	-
C3Z06_RS24370	5194410..5195321	+	912	WP_003105643.1	TIGR03749 family integrating conjugative element protein	-
C3Z06_RS24375	5195311..5196729	+	1419	WP_042178570.1	TIGR03752 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5142477..5242120	99643

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21644.79 Da
• Isoelectric Point: 5.6172

>T95089 WP_003109777.1 NZ_CP026544:c5191654-5191079 [Cupriavidus metallidurans]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIEVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T95089 NZ_CP026544:c5191654-5191079 [Cupriavidus metallidurans]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACCGGGCAATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTGCCCGATCCGGACGACCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGGGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTACTGGATCTGGACGCGGCCGCCGTGGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGAGGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT95089 WP_003050245.1 NZ_CP026544:c5192121-5191651 [Cupriavidus metallidurans]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT95089 NZ_CP026544:c5192121-5191651 [Cupriavidus metallidurans]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTGAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTCGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68