Detailed information of TA system TA095077

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3133941..3134858
• Replicon: chromosome
• Accession: NZ_CP026533
• Organism: Bacillus velezensis strain DKU_NT_04

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: C3438_RS16440
• Protein ID: WP_007407256.1
• Coordinates: 3134112..3134858 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: C3438_RS16435
• Protein ID: WP_003154807.1
• Coordinates: 3133941..3134111 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3438_RS16390	3129147..3129659	+	513	WP_017417605.1	sigma-70 family RNA polymerase sigma factor	-
C3438_RS16395	3129772..3130431	+	660	WP_104843289.1	terminase	-
C3438_RS16400	3130329..3130796	+	468	Protein_3128	hypothetical protein	-
C3438_RS16405	3130809..3131180	+	372	WP_017417608.1	hypothetical protein	-
C3438_RS16410	3131185..3131382	+	198	Protein_3130	XkdX family protein	-
C3438_RS16415	3131439..3132200	+	762	WP_063636818.1	hypothetical protein	-
C3438_RS16420	3132252..3132515	+	264	WP_021493821.1	hemolysin XhlA family protein	-
C3438_RS16425	3132529..3132792	+	264	WP_003154813.1	phage holin	-
C3438_RS16430	3132806..3133684	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
C3438_RS16435	3133941..3134111	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
C3438_RS16440	3134112..3134858	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
C3438_RS16445	3134962..3135960	-	999	WP_017417610.1	inorganic phosphate transporter	-
C3438_RS16450	3135973..3136590	-	618	WP_104843290.1	DUF47 domain-containing protein	-
C3438_RS16455	3136877..3138193	-	1317	WP_007610842.1	amino acid permease	-
C3438_RS16460	3138515..3139465	+	951	WP_007610844.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T95077 WP_007407256.1 NZ_CP026533:c3134858-3134112 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T95077 NZ_CP026533:c3134858-3134112 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGAAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACTGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT95077 WP_003154807.1 NZ_CP026533:c3134111-3133941 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT95077 NZ_CP026533:c3134111-3133941 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14