Detailed information of TA system TA095076

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2309450..2310087
• Replicon: chromosome
• Accession: NZ_CP026533
• Organism: Bacillus velezensis strain DKU_NT_04

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: C3438_RS11960
• Protein ID: WP_003156187.1
• Coordinates: 2309737..2310087 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: C3438_RS11955
• Protein ID: WP_003156188.1
• Coordinates: 2309450..2309731 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3438_RS11935	2305815..2306414	-	600	WP_104843104.1	rhomboid family intramembrane serine protease	-
C3438_RS11940	2306507..2306872	+	366	WP_104843105.1	holo-ACP synthase	-
C3438_RS11945	2307037..2308044	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
C3438_RS11950	2308161..2309330	+	1170	WP_104843106.1	alanine racemase	-
C3438_RS11955	2309450..2309731	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
C3438_RS11960	2309737..2310087	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
C3438_RS11965	2310205..2311026	+	822	WP_003156182.1	STAS domain-containing protein	-
C3438_RS11970	2311031..2311396	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
C3438_RS11975	2311399..2311800	+	402	WP_017419223.1	anti-sigma regulatory factor	-
C3438_RS11980	2311812..2312819	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
C3438_RS11985	2312883..2313212	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
C3438_RS11990	2313209..2313691	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
C3438_RS11995	2313657..2314445	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
C3438_RS12000	2314445..2315047	+	603	WP_017419222.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T95076 WP_003156187.1 NZ_CP026533:2309737-2310087 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T95076 NZ_CP026533:2309737-2310087 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT95076 WP_003156188.1 NZ_CP026533:2309450-2309731 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT95076 NZ_CP026533:2309450-2309731 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5