95068

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 668988..669516 Replicon chromosome
Accession NZ_CP026532
Organism Vibrio cholerae strain FORC_076

Toxin (Protein)


Gene name relE Uniprot ID H9L4R3
Locus tag FORC76_RS17315 Protein ID WP_000221352.1
Coordinates 669226..669516 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID H9L4T4
Locus tag FORC76_RS17310 Protein ID WP_000213183.1
Coordinates 668988..669236 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC76_RS17250 664021..664476 - 456 WP_029628723.1 GNAT family N-acetyltransferase -
FORC76_RS17255 664791..664988 - 198 WP_080006741.1 DUF3709 domain-containing protein -
FORC76_RS17260 664982..665143 - 162 Protein_639 hypothetical protein -
FORC76_RS17265 665245..666213 - 969 WP_002033239.1 hypothetical protein -
FORC76_RS17270 666413..666658 + 246 WP_001260801.1 type II toxin-antitoxin system CcdA family antitoxin -
FORC76_RS17275 666658..666975 + 318 WP_000077274.1 CcdB family protein -
FORC76_RS17285 667224..667457 - 234 WP_084998720.1 DUF3709 domain-containing protein -
FORC76_RS17290 667478..667639 - 162 Protein_644 DUF3709 domain-containing protein -
FORC76_RS17300 667730..668155 - 426 WP_000403002.1 GNAT family N-acetyltransferase -
FORC76_RS17305 668423..668701 - 279 WP_128974295.1 DUF3709 domain-containing protein -
FORC76_RS17310 668988..669236 + 249 WP_000213183.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
FORC76_RS17315 669226..669516 + 291 WP_000221352.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FORC76_RS17325 669647..670048 - 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
FORC76_RS17330 670048..670218 - 171 WP_001080654.1 hypothetical protein -
FORC76_RS17340 670531..670809 - 279 WP_080285018.1 DUF3709 domain-containing protein -
FORC76_RS17345 671096..671278 - 183 WP_032480896.1 DUF645 family protein -
FORC76_RS17350 671574..672062 - 489 Protein_653 dual specificity protein phosphatase family protein -
FORC76_RS17355 672182..672556 - 375 WP_001221132.1 hypothetical protein -
FORC76_RS17360 672679..673143 - 465 WP_032480879.1 hypothetical protein -
FORC76_RS19295 673153..673224 - 72 Protein_656 acetyltransferase -
FORC76_RS17365 673293..673970 - 678 WP_002033254.1 FMN-binding negative transcriptional regulator -
FORC76_RS19300 674120..674216 - 97 Protein_658 DUF645 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island blaCARB-9 - 564019..691460 127441
- inside Integron - - 566037..688204 122167


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11247.23 Da        Isoelectric Point: 10.4678

>T95068 WP_000221352.1 NZ_CP026532:669226-669516 [Vibrio cholerae]
MTYKLEFKKSALKEWKKLAVPLQQQFKKKLIERLENPHVPSAKLSGAENIYKIKLRQSGYRLVYQVENDIIVVTVLAVGK
RERSEVYTKALQRLDD

Download         Length: 291 bp

>T95068 NZ_CP026532:669226-669516 [Vibrio cholerae]
ATGACCTATAAGCTTGAGTTCAAAAAGAGTGCTTTGAAGGAATGGAAAAAGTTAGCCGTTCCACTCCAACAGCAGTTCAA
GAAGAAACTAATAGAACGCCTTGAAAATCCACATGTTCCATCAGCCAAACTGTCTGGCGCTGAGAACATTTATAAAATAA
AACTTCGGCAGTCTGGTTATCGCTTAGTTTATCAAGTTGAAAATGACATCATCGTAGTCACTGTTTTAGCTGTCGGTAAA
CGAGAACGCAGCGAAGTTTACACTAAAGCGCTGCAACGGCTAGACGACTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8937.28 Da        Isoelectric Point: 4.1079

>AT95068 WP_000213183.1 NZ_CP026532:668988-669236 [Vibrio cholerae]
MTTRILADVAASITEFKANPMKVATSAFGAPVAVLNRNEPAFYCVPASTYEIMMDKLEDLELLAIAKERLSEDSVSVNID
DL

Download         Length: 249 bp

>AT95068 NZ_CP026532:668988-669236 [Vibrio cholerae]
ATGACCACTAGAATCCTAGCTGATGTTGCAGCAAGCATTACCGAATTTAAAGCAAACCCAATGAAAGTTGCAACTAGCGC
CTTCGGTGCTCCTGTTGCTGTACTCAACCGAAATGAGCCGGCTTTCTACTGCGTCCCTGCAAGCACCTACGAGATCATGA
TGGATAAGCTTGAAGATCTTGAGCTACTGGCTATTGCTAAAGAACGCTTAAGTGAAGACAGCGTATCGGTAAATATCGAT
GACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB H9L4R3

Antitoxin

Source ID Structure
AlphaFold DB A0A366A3W8

References