Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /DUF1778(antitoxin)
Location 588417..589216 Replicon chromosome
Accession NZ_CP026532
Organism Vibrio cholerae strain FORC_076

Toxin (Protein)


Gene name - Uniprot ID D7HHI3
Locus tag FORC76_RS16425 Protein ID WP_000118353.1
Coordinates 588417..588950 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A0K9UER4
Locus tag FORC76_RS16430 Protein ID WP_000179600.1
Coordinates 588947..589216 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC76_RS16365 583556..583737 - 182 Protein_500 DUF645 family protein -
FORC76_RS16370 583907..583975 - 69 Protein_501 glutathione S-transferase family protein -
FORC76_RS16375 584041..584436 - 396 WP_001923862.1 DUF3465 domain-containing protein -
FORC76_RS16380 584724..584957 - 234 WP_001990688.1 DUF3709 domain-containing protein -
FORC76_RS16385 584978..585139 - 162 WP_113625331.1 DUF3709 domain-containing protein -
FORC76_RS16390 585230..585766 - 537 WP_084988691.1 GNAT family N-acetyltransferase -
FORC76_RS16395 585915..586417 - 503 Protein_506 DinB family protein -
FORC76_RS16405 586645..586826 - 182 Protein_507 DUF645 family protein -
FORC76_RS16410 587252..587575 - 324 WP_080006739.1 DUF3709 domain-containing protein -
FORC76_RS16415 587762..588268 - 507 WP_000403020.1 GNAT family N-acetyltransferase -
FORC76_RS16425 588417..588950 - 534 WP_000118353.1 GNAT family N-acetyltransferase Toxin
FORC76_RS16430 588947..589216 - 270 WP_000179600.1 DUF1778 domain-containing protein Antitoxin
FORC76_RS16435 589570..589848 - 279 WP_164971223.1 DUF3709 domain-containing protein -
FORC76_RS16445 590134..590172 - 39 WP_110168446.1 hypothetical protein -
FORC76_RS16450 590191..590316 - 126 WP_080006744.1 DUF645 family protein -
FORC76_RS16460 590611..591528 - 918 WP_000627640.1 hypothetical protein -
FORC76_RS19200 591681..592592 - 912 WP_032480859.1 hypothetical protein -
FORC76_RS16465 592926..593123 - 198 WP_080006741.1 DUF3709 domain-containing protein -
FORC76_RS16470 593117..593278 - 162 Protein_518 hypothetical protein -
FORC76_RS16475 593367..593885 - 519 WP_000699012.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island blaCARB-9 - 564019..691460 127441
- inside Integron - - 566037..688204 122167


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(97-153)

Antitoxin

Vibrio phage ICP1, Orf50

(5-81)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 20192.48 Da        Isoelectric Point: 9.1426

>T95062 WP_000118353.1 NZ_CP026532:c588950-588417 [Vibrio cholerae]
VSWGKEFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSAQPLLNQKFAICAFYSVAPSSISRETLPT
QLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKVSLIRALKYLWEVNHHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMFLPMKTVEQLFNQ

Download         Length: 534 bp

>T95062 NZ_CP026532:c588950-588417 [Vibrio cholerae]
GTGAGTTGGGGCAAAGAGTTTGTAGAACTTAGTAAATCAAAGCATGACCGAGACTCATTTGACTGTGGTGAGCAAGAGCT
AAACACATTTATCAAAACTCAAGCTGCAAAGCACATGCAAGCAGGTATTAGCCGAACCATGGTTTTACCTAGTGCTCAAC
CACTGTTAAATCAAAAATTCGCTATTTGTGCCTTTTATAGTGTTGCACCGAGCTCAATCAGCCGAGAAACATTGCCGACA
CAATTAGCAAAGAAACTACCTAGGTATCCAATCCCTGTTTTTCTGTTGGCTCAGTTAGCTGTACACAAAGAATTTCACGG
TTCTGGACTTGGAAAGGTTAGTCTAATTCGTGCGCTGAAGTACTTGTGGGAAGTGAATCATCACATGAGGGCATACGCAA
TAGTTGTGGATTGTCTGACAGATTCTGCTCAAGCGTTCTATACGAAGTTCGGCTTTGAAGTATTGTGCGAACATAATGAG
CGTATTCGTATGTTCTTACCGATGAAAACGGTAGAACAACTTTTTAATCAATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9924.49 Da        Isoelectric Point: 4.9107

>AT95062 WP_000179600.1 NZ_CP026532:c589216-588947 [Vibrio cholerae]
MATARLDIRLDEEIKAKAEKASALLGLKSLTEYVVRLMDEDATHVIEEHESIMVKDSVFDEFMAACNKVKAPNQALLEAV
KFTEKSGIK

Download         Length: 270 bp

>AT95062 NZ_CP026532:c589216-588947 [Vibrio cholerae]
ATGGCAACGGCACGTTTAGATATCCGCCTTGATGAAGAGATCAAGGCAAAAGCCGAAAAGGCATCGGCTTTACTTGGTTT
AAAAAGTCTCACTGAATATGTGGTGAGACTTATGGACGAAGATGCGACTCATGTTATTGAGGAGCACGAAAGTATTATGG
TTAAAGACAGTGTTTTTGACGAGTTTATGGCTGCGTGTAATAAAGTAAAAGCACCGAATCAAGCTTTGCTTGAAGCTGTT
AAATTTACAGAGAAGAGCGGTATCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D7HHI3

Antitoxin

Source ID Structure
AlphaFold DB A0A0K9UER4

References