Detailed information of TA system TA095053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1431107..1431714
• Replicon: plasmid pSymA
• Accession: NZ_CP026526
• Organism: Sinorhizobium meliloti strain AK21

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C3L21_RS25890
• Protein ID: WP_017264738.1
• Coordinates: 1431107..1431361 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C3L21_RS25895
• Protein ID: WP_040120685.1
• Coordinates: 1431358..1431714 (+)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3L21_RS25865	1426508..1427212	-	705	WP_158528728.1	haloacid dehalogenase type II	-
C3L21_RS25870	1427303..1428175	+	873	WP_010968043.1	LysR family transcriptional regulator	-
C3L21_RS25875	1428226..1428381	-	156	WP_153320124.1	hypothetical protein	-
C3L21_RS36200	1428467..1428625	-	159	WP_017276730.1	hypothetical protein	-
C3L21_RS25880	1428749..1429789	-	1041	WP_040120687.1	NAD(P)H-binding protein	-
C3L21_RS25885	1429878..1430765	+	888	WP_040120686.1	LysR family transcriptional regulator	-
C3L21_RS25890	1431107..1431361	+	255	WP_017264738.1	type II toxin-antitoxin system HicA family toxin	Toxin
C3L21_RS25895	1431358..1431714	+	357	WP_040120685.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C3L21_RS25900	1432086..1432742	-	657	WP_010968046.1	SGNH/GDSL hydrolase family protein	-
C3L21_RS25905	1432772..1433722	-	951	WP_010968047.1	ABC transporter permease	-
C3L21_RS25910	1433725..1434696	-	972	WP_040120683.1	ABC transporter permease	-
C3L21_RS25915	1434713..1436206	-	1494	WP_010968049.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB / htpB	1..1506823	1506823

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9294.62 Da
• Isoelectric Point: 9.8124

>T95053 WP_017264738.1 NZ_CP026526:1431107-1431361 [Sinorhizobium meliloti]
MNSKQRKTLAAVFKDPASGTIEWAAIENLLVAAGADVIEGNGSRVKFEKDGIIASFHRPHPDKEAKRYQVRDAREFLMQI
GVTP

Nucleotide

• Length: 255 bp

>T95053 NZ_CP026526:1431107-1431361 [Sinorhizobium meliloti]
ATGAACAGCAAGCAGAGAAAGACCCTCGCGGCCGTATTCAAGGACCCGGCCTCGGGAACGATTGAATGGGCCGCTATAGA
GAATCTGCTTGTGGCTGCCGGAGCGGACGTCATTGAGGGGAACGGTTCCCGCGTTAAGTTTGAAAAAGACGGCATCATTG
CGAGCTTTCATCGACCTCATCCGGACAAGGAAGCAAAGCGTTATCAGGTGCGCGATGCTCGCGAGTTCCTGATGCAGATT
GGAGTAACGCCATGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13178.82 Da
• Isoelectric Point: 5.2288

>AT95053 WP_040120685.1 NZ_CP026526:1431358-1431714 [Sinorhizobium meliloti]
MNTMTYNGYHARIEFDAEDEVFFGKIAGISDVIGFHGDSVAELKKAFHEAVDDYLETCRKIGKEPQRPYSGKMMFRVAPE
VHRRAALAAELSGKSLNQWAEEVLEEAAGHFAEARLSA

Nucleotide

• Length: 357 bp

>AT95053 NZ_CP026526:1431358-1431714 [Sinorhizobium meliloti]
ATGAACACCATGACCTACAACGGGTATCACGCTCGCATCGAATTCGACGCTGAGGACGAAGTGTTTTTCGGCAAGATTGC
AGGTATCTCCGATGTAATCGGCTTTCACGGTGACAGTGTCGCTGAACTCAAGAAAGCCTTCCATGAGGCGGTCGATGACT
ACCTTGAGACCTGCAGGAAAATCGGCAAAGAGCCTCAGCGACCCTATTCTGGGAAGATGATGTTTCGTGTCGCTCCCGAA
GTCCATCGCCGAGCAGCTCTTGCTGCTGAGCTTTCGGGAAAGAGTCTCAACCAGTGGGCAGAAGAGGTGCTGGAAGAAGC
CGCCGGCCATTTTGCCGAGGCGCGCCTGTCGGCGTAG