Detailed information of TA system TA095051

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 898155..898840
• Replicon: plasmid pSymA
• Accession: NZ_CP026526
• Organism: Sinorhizobium meliloti strain AK21

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C3L21_RS23230
• Protein ID: WP_010967510.1
• Coordinates: 898155..898574 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: C3L21_RS23235
• Protein ID: WP_041169972.1
• Coordinates: 898571..898840 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3L21_RS23215	893249..893896	+	648	Protein_874	autoinducer binding domain-containing protein	-
C3L21_RS23220	894423..895841	-	1419	WP_158528697.1	ISNCY family transposase	-
C3L21_RS23225	896135..897562	-	1428	WP_015241193.1	ISNCY family transposase	-
C3L21_RS23230	898155..898574	-	420	WP_010967510.1	type II toxin-antitoxin system VapC family toxin	Toxin
C3L21_RS23235	898571..898840	-	270	WP_041169972.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C3L21_RS23240	898949..899152	+	204	Protein_879	hypothetical protein	-
C3L21_RS23245	899215..900090	-	876	WP_015445434.1	LysR family transcriptional regulator	-
C3L21_RS36160	901100..901270	+	171	WP_162471696.1	hypothetical protein	-
C3L21_RS23260	901585..901977	+	393	WP_010967515.1	hypothetical protein	-
C3L21_RS23265	902034..902414	-	381	WP_010967516.1	transcriptional regulator	-
C3L21_RS23270	902432..902743	-	312	WP_003526630.1	type II toxin-antitoxin system HigB family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB / htpB	1..1506823	1506823
	-	flank	IS/Tn	-	-	902910..904172	1262

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (18-55)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14785.05 Da
• Isoelectric Point: 4.8837

>T95051 WP_010967510.1 NZ_CP026526:c898574-898155 [Sinorhizobium meliloti]
VSRLYMLDTNIVSELARNPQGAVTKRIAEVGPEAVCVSIITAAELRYGCAKKGSPKLLAQIEAILGSMQVLALDVPADAE
YGNIRAELETAGKPIGPNDLFIAAHACVLGAVLVTVNSSEFTRVRDLKVENWLDFTSSG

Nucleotide

• Length: 420 bp

>T95051 NZ_CP026526:c898574-898155 [Sinorhizobium meliloti]
GTGAGCAGGCTGTACATGCTGGACACCAATATCGTGTCCGAGCTTGCGCGAAACCCACAGGGGGCTGTCACTAAACGCAT
TGCCGAGGTAGGACCGGAGGCAGTCTGCGTCAGCATCATTACGGCGGCAGAGTTACGCTATGGATGCGCCAAGAAGGGAT
CACCGAAGCTACTGGCGCAAATCGAGGCCATTCTTGGAAGTATGCAAGTGCTTGCGCTCGATGTGCCTGCTGATGCCGAA
TATGGCAACATCCGGGCTGAACTGGAGACCGCTGGTAAGCCCATCGGTCCGAATGACCTGTTCATCGCCGCTCATGCCTG
TGTGCTTGGAGCGGTGCTGGTAACGGTCAACAGTAGCGAGTTCACACGCGTTCGTGATCTCAAGGTCGAGAACTGGCTGG
ATTTCACTTCTTCGGGTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10129.55 Da
• Isoelectric Point: 4.4596

>AT95051 WP_041169972.1 NZ_CP026526:c898840-898571 [Sinorhizobium meliloti]
MPQLRHDEPPPPREAKLFRNNRSQAVRIPADFELPGDRVMIHRDGDRLIIEPVLRKNLLEVLAGLEPLGSEDEFPDVDET
LLPGKDVDL

Nucleotide

• Length: 270 bp

>AT95051 NZ_CP026526:c898840-898571 [Sinorhizobium meliloti]
ATGCCCCAACTCCGACACGACGAACCACCACCGCCAAGGGAAGCCAAGCTCTTCCGAAATAATAGAAGTCAGGCTGTACG
TATCCCCGCTGATTTTGAGCTTCCCGGCGATCGGGTGATGATCCATCGGGATGGAGATCGGCTGATAATCGAGCCGGTGC
TCCGCAAGAACTTGCTGGAAGTACTGGCAGGTTTGGAGCCGCTCGGGTCTGAAGATGAGTTCCCTGACGTTGATGAAACC
CTGCTGCCGGGGAAGGATGTCGACCTGTGA