Detailed information of TA system TA095049

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3747778..3748439
• Replicon: chromosome
• Accession: NZ_CP026525
• Organism: Sinorhizobium meliloti strain AK21

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: H0G400
• Locus tag: C3L21_RS18385
• Protein ID: WP_003531794.1
• Coordinates: 3747778..3748182 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: H0G3Z9
• Locus tag: C3L21_RS18390
• Protein ID: WP_003531793.1
• Coordinates: 3748179..3748439 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3L21_RS18370	3743374..3744486	+	1113	WP_003535893.1	3-isopropylmalate dehydrogenase	-
C3L21_RS18375	3744615..3745322	+	708	WP_127521406.1	phosphatase PAP2 family protein	-
C3L21_RS18380	3745423..3747312	-	1890	WP_003535895.1	MHS family MFS transporter	-
C3L21_RS18385	3747778..3748182	-	405	WP_003531794.1	type II toxin-antitoxin system VapC family toxin	Toxin
C3L21_RS18390	3748179..3748439	-	261	WP_003531793.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C3L21_RS18400	3748852..3749886	+	1035	WP_003531790.1	aspartate-semialdehyde dehydrogenase	-
C3L21_RS18405	3750059..3750880	+	822	WP_003531785.1	lytic transglycosylase family protein	-
C3L21_RS18410	3751227..3751910	-	684	WP_010970569.1	carbonic anhydrase	-
C3L21_RS18415	3752084..3752965	-	882	WP_015008003.1	pyridoxal kinase PdxY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (8-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14480.69 Da
• Isoelectric Point: 6.7257

>T95049 WP_003531794.1 NZ_CP026525:c3748182-3747778 [Sinorhizobium meliloti]
VISHILDTSAVIALIGRKSDALVTRVLHSPQGIIGLPSVVAYELYFGAQKSAKAQHNLETLRLLMADFPILDFDRNDAFV
AGEIRAALAAKGTPIGPYDVLIAGQAKARGLTLVTNNVGEFNRVENLRVEDWSL

Nucleotide

• Length: 405 bp

>T95049 NZ_CP026525:c3748182-3747778 [Sinorhizobium meliloti]
GTGATCTCCCATATTCTCGACACCAGCGCCGTCATCGCTTTGATCGGGCGCAAATCGGATGCATTGGTCACCCGTGTACT
CCATAGCCCTCAGGGCATTATCGGGCTCCCGTCGGTCGTCGCGTATGAACTCTATTTTGGAGCGCAGAAAAGCGCGAAGG
CCCAACACAATCTCGAAACCCTGCGTTTGTTGATGGCGGATTTTCCGATCCTCGACTTCGACCGGAACGATGCCTTTGTC
GCAGGCGAGATCCGGGCCGCCCTTGCCGCGAAGGGTACGCCCATCGGACCTTATGACGTGCTGATTGCGGGACAGGCCAA
AGCCCGTGGATTGACGCTCGTCACCAACAATGTCGGCGAGTTCAATAGGGTTGAGAACCTGCGCGTGGAGGATTGGTCGC
TCTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9913.20 Da
• Isoelectric Point: 5.1253

>AT95049 WP_003531793.1 NZ_CP026525:c3748439-3748179 [Sinorhizobium meliloti]
MPHLARVFQSGNSQAVRLPKEFRFNVDRVEITQEGDALILRPHVEQGEQWSSLKAALARGMSEDFMMCGREQPEQQDRPE
LDAVFR

Nucleotide

• Length: 261 bp

>AT95049 NZ_CP026525:c3748439-3748179 [Sinorhizobium meliloti]
ATGCCCCATCTTGCACGGGTGTTCCAGTCCGGAAATTCGCAGGCCGTCCGCCTGCCTAAGGAATTCCGCTTCAACGTCGA
TCGCGTCGAAATCACGCAGGAAGGCGACGCGCTCATTCTCCGCCCGCACGTCGAGCAAGGCGAGCAGTGGTCATCGCTGA
AGGCGGCACTTGCCCGTGGCATGAGTGAAGACTTCATGATGTGCGGGCGCGAGCAGCCGGAACAGCAGGATCGGCCGGAA
CTCGACGCGGTATTTCGGTGA

Structures

Toxin

• AlphaFold DB: H0G400

Antitoxin

• AlphaFold DB: H0G3Z9