Detailed information of TA system TA095047

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 2831762..2832435
• Replicon: chromosome
• Accession: NZ_CP026525
• Organism: Sinorhizobium meliloti strain AK21

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C3L21_RS14105
• Protein ID: WP_010969926.1
• Coordinates: 2832031..2832435 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: H0GA10
• Locus tag: C3L21_RS14100
• Protein ID: WP_003536345.1
• Coordinates: 2831762..2832034 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3L21_RS14080	2827753..2829393	-	1641	WP_003536336.1	ABC transporter substrate-binding protein	-
C3L21_RS14085	2829441..2830496	-	1056	WP_014529838.1	dipeptidase	-
C3L21_RS14090	2830676..2831017	-	342	WP_003536339.1	EamA family transporter	-
C3L21_RS14095	2831065..2831625	-	561	WP_010969925.1	TetR/AcrR family transcriptional regulator	-
C3L21_RS14100	2831762..2832034	+	273	WP_003536345.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C3L21_RS14105	2832031..2832435	+	405	WP_010969926.1	type II toxin-antitoxin system VapC family toxin	Toxin
C3L21_RS14110	2832563..2832955	+	393	WP_003536349.1	globin	-
C3L21_RS14115	2832961..2833335	+	375	WP_010969927.1	DUF423 domain-containing protein	-
C3L21_RS14120	2833458..2833718	+	261	WP_010969928.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
C3L21_RS14125	2833833..2834147	-	315	WP_003536354.1	antibiotic biosynthesis monooxygenase	-
C3L21_RS14130	2834160..2834426	-	267	WP_010969929.1	hypothetical protein	-
C3L21_RS14135	2834479..2834892	-	414	WP_003536357.1	DUF2325 domain-containing protein	-
C3L21_RS14140	2835100..2836026	-	927	WP_010969930.1	energy transducer TonB	-
C3L21_RS14145	2836067..2836501	-	435	WP_010969931.1	hypothetical protein	-
C3L21_RS14150	2836731..2836871	+	141	WP_010969932.1	hemin uptake protein HemP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (16-54)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14747.98 Da
• Isoelectric Point: 5.1974

>T95047 WP_010969926.1 NZ_CP026525:2832031-2832435 [Sinorhizobium meliloti]
MNGYLLDTNIISDVIHNPFGPAAQRIERIGPKEIYTSIVVASELRYGCAKKGSAKLLAKVESLLEIVPVLPLDIPADTRY
GSIRAELESLGQTIGSNDLLIAAHAYALDLTLVTDNIREFSRVRGLSLENWLER

Nucleotide

• Length: 405 bp

>T95047 NZ_CP026525:2832031-2832435 [Sinorhizobium meliloti]
TTGAACGGCTATCTTCTCGATACGAACATCATCAGCGACGTCATTCACAATCCGTTCGGACCGGCTGCGCAACGCATCGA
ACGGATCGGCCCGAAGGAGATTTACACCAGCATCGTCGTCGCGTCCGAGTTGCGGTATGGTTGCGCAAAAAAGGGATCGG
CGAAGTTGCTCGCCAAGGTCGAAAGCTTGCTGGAAATCGTCCCGGTGCTGCCATTGGATATTCCGGCCGACACCCGATAT
GGCAGCATTCGTGCGGAATTGGAGTCTCTGGGGCAGACCATCGGCTCCAACGATCTGTTGATTGCCGCGCATGCATATGC
GCTGGACCTGACGCTGGTGACGGACAATATCCGTGAGTTCAGCCGCGTTCGTGGCCTTAGTCTGGAAAACTGGCTTGAGC
GATAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10334.02 Da
• Isoelectric Point: 6.3950

>AT95047 WP_003536345.1 NZ_CP026525:2831762-2832034 [Sinorhizobium meliloti]
MPVPLPSSRPKEVKLFRNNRSQAVRIPAEFELPGDRVLIRREGTRLIIEPIARPADIVELLAEWKKEAPLGPEDRFPDVE
DIPARPEKIF

Nucleotide

• Length: 273 bp

>AT95047 NZ_CP026525:2831762-2832034 [Sinorhizobium meliloti]
ATGCCCGTCCCGTTGCCGTCATCGCGACCTAAAGAAGTAAAGCTGTTCCGCAATAACCGCAGCCAGGCAGTCCGTATTCC
TGCGGAGTTCGAGTTGCCTGGAGACCGTGTGTTGATCCGTCGCGAAGGAACGAGACTCATTATCGAACCGATAGCCAGGC
CAGCCGATATCGTGGAGCTTCTCGCGGAGTGGAAGAAGGAAGCTCCCCTCGGACCTGAAGACCGGTTCCCGGATGTTGAG
GATATACCCGCCCGGCCGGAGAAAATCTTTTGA

Structures

Antitoxin

• AlphaFold DB: H0GA10