95043

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 525109..525674 Replicon chromosome
Accession NZ_CP026525
Organism Sinorhizobium meliloti strain AK21

Toxin (Protein)


Gene name brnT Uniprot ID H0G3P4
Locus tag C3L21_RS02445 Protein ID WP_003531565.1
Coordinates 525429..525674 (-) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID H0G3P5
Locus tag C3L21_RS02440 Protein ID WP_003531566.1
Coordinates 525109..525432 (-) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3L21_RS02420 520972..521772 + 801 WP_003531572.1 TVP38/TMEM64 family protein -
C3L21_RS02425 521795..523216 + 1422 WP_003531570.1 NAD(P)/FAD-dependent oxidoreductase -
C3L21_RS02430 523291..524811 + 1521 WP_010968584.1 HAMP domain-containing histidine kinase -
C3L21_RS02440 525109..525432 - 324 WP_003531566.1 BrnA antitoxin family protein Antitoxin
C3L21_RS02445 525429..525674 - 246 WP_003531565.1 BrnT family toxin Toxin
C3L21_RS02450 526109..526822 + 714 WP_003531563.1 replicative DNA helicase -
C3L21_RS02455 527009..527704 - 696 WP_015007123.1 TetR/AcrR family transcriptional regulator C-terminal domain-containing protein -
C3L21_RS02460 527896..528384 + 489 WP_003531560.1 RidA family protein -
C3L21_RS02465 528593..529210 + 618 WP_003531558.1 hypothetical protein -
C3L21_RS02470 529298..530443 + 1146 WP_003531550.1 alpha-hydroxy-acid oxidizing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-77)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-101)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 8990.48 Da        Isoelectric Point: 9.7323

>T95043 WP_003531565.1 NZ_CP026525:c525674-525429 [Sinorhizobium meliloti]
MKVTYDETKRQINIGKHGYDIAELDMEFFASSVVVPAKEGRFMAIGVLRNGVIAVVFARLGSEGISIISMRDASRKERSL
L

Download         Length: 246 bp

>T95043 NZ_CP026525:c525674-525429 [Sinorhizobium meliloti]
ATGAAGGTCACCTATGACGAAACAAAGCGCCAGATCAACATAGGAAAGCACGGCTATGATATCGCCGAACTCGACATGGA
GTTCTTCGCTTCGTCTGTGGTGGTCCCAGCCAAGGAAGGCCGCTTCATGGCTATCGGCGTTTTGCGCAATGGCGTTATCG
CCGTAGTCTTCGCCCGGCTTGGCTCGGAAGGCATTTCGATTATCTCCATGCGTGACGCCAGCAGGAAGGAAAGGAGCTTA
TTATGA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 11933.51 Da        Isoelectric Point: 10.2830

>AT95043 WP_003531566.1 NZ_CP026525:c525432-525109 [Sinorhizobium meliloti]
MTSLSRRIRHISDKEEAAIQRKIASDPDAPEATDEQLAKAKPFAEAFPDLAESIKRARGRPAVENPRQQISIRLDPDVIE
HYKATGKGWQSRMNDDLRKAAGLKKAG

Download         Length: 324 bp

>AT95043 NZ_CP026525:c525432-525109 [Sinorhizobium meliloti]
ATGACCAGCCTATCCCGCCGCATCCGCCATATCTCCGACAAGGAGGAGGCAGCTATCCAACGCAAGATAGCTAGTGACCC
AGACGCTCCTGAGGCCACCGACGAACAACTAGCCAAAGCTAAGCCATTCGCCGAAGCCTTCCCAGACCTGGCCGAGAGCA
TCAAGCGCGCCAGGGGCCGTCCAGCCGTTGAGAACCCTAGGCAGCAGATATCCATTCGCCTCGATCCGGATGTCATCGAG
CACTACAAGGCAACCGGCAAAGGCTGGCAGAGCCGGATGAATGACGACCTTAGAAAGGCTGCCGGATTGAAGAAGGCAGG
TTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB H0G3P4

Antitoxin

Source ID Structure
AlphaFold DB H0G3P5

References