Detailed information of TA system TA095029

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4591062..4591765
• Replicon: chromosome
• Accession: NZ_CP026495
• Organism: Klebsiella pneumoniae strain 616

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A939NMR5
• Locus tag: C2861_RS22715
• Protein ID: WP_071994632.1
• Coordinates: 4591062..4591403 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A939NIK9
• Locus tag: C2861_RS22720
• Protein ID: WP_032434296.1
• Coordinates: 4591424..4591765 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2861_RS22705	4587377..4588246	+	870	WP_023317468.1	HNH endonuclease	-
C2861_RS22710	4588837..4590870	+	2034	WP_050598589.1	hypothetical protein	-
C2861_RS22715	4591062..4591403	-	342	WP_071994632.1	TA system toxin CbtA family protein	Toxin
C2861_RS22720	4591424..4591765	-	342	WP_032434296.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C2861_RS22725	4591776..4592318	-	543	WP_032434298.1	DNA repair protein RadC	-
C2861_RS22730	4592331..4592771	-	441	WP_032434300.1	antirestriction protein	-
C2861_RS22735	4592802..4593623	-	822	WP_032434301.1	DUF945 domain-containing protein	-
C2861_RS22740	4593743..4594216	-	474	WP_032434303.1	hypothetical protein	-
C2861_RS22745	4594288..4594740	-	453	WP_032410767.1	hypothetical protein	-
C2861_RS22750	4594776..4595492	-	717	WP_032434305.1	WYL domain-containing protein	-
C2861_RS22755	4595736..4596610	-	875	Protein_4354	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4579359..4625032	45673

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12789.77 Da
• Isoelectric Point: 9.6552

>T95029 WP_071994632.1 NZ_CP026495:c4591403-4591062 [Klebsiella pneumoniae]
MKTLPATTPQAVTLCLSSVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRKGF
NWQEHSPYLRAVDILRARQATGLLRRSRKNAVR

Nucleotide

• Length: 342 bp

>T95029 NZ_CP026495:c4591403-4591062 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGTGACGCTATGTCTGTCGTCTGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAAGGGATTC
AACTGGCAAGAACACTCCCCATATCTGCGGGCAGTTGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGCGCCGAAG
CCGTAAAAATGCTGTACGTTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12893.40 Da
• Isoelectric Point: 5.9612

>AT95029 WP_032434296.1 NZ_CP026495:c4591765-4591424 [Klebsiella pneumoniae]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGEFSNAESLKLDEVFPHFISQMESMLTTGEMNPRH
AHFFTLYHNGFTCEADTLGSYGYVYIAVYPTQR

Nucleotide

• Length: 342 bp

>AT95029 NZ_CP026495:c4591765-4591424 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGACTTCACTATCTGGCTGACCGGGCCAGCATTACCGGTGAATTCAGCAACGCAGAATCTT
TGAAGTTGGATGAGGTATTCCCACATTTTATCAGCCAGATGGAGTCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTTTTTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA