Detailed information of TA system TA095003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 107328..107863
• Replicon: plasmid pHS13-1-IncF
• Accession: NZ_CP026494
• Organism: Escherichia coli strain HS13-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0VJJ7
• Locus tag: C3K24_RS28530
• Protein ID: WP_000222760.1
• Coordinates: 107328..107615 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0VIP2
• Locus tag: C3K24_RS28535
• Protein ID: WP_001132900.1
• Coordinates: 107612..107863 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3K24_RS28490	102911..104132	-	1222	Protein_99	ISL3 family transposase	-
C3K24_RS28500	104232..104596	+	365	Protein_100	IS1 family transposase	-
C3K24_RS28505	104651..105433	-	783	WP_001442137.1	IS21-like element IS100kyp family helper ATPase IstB	-
C3K24_RS28510	105430..106452	-	1023	WP_001310017.1	IS21-like element IS100 family transposase	-
C3K24_RS28520	106541..106888	+	348	Protein_103	IS1-like element IS1A family transposase	-
C3K24_RS28530	107328..107615	-	288	WP_000222760.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C3K24_RS28535	107612..107863	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C3K24_RS28540	108758..110509	-	1752	Protein_106	Tn3-like element TnAs1 family transposase	-
C3K24_RS28545	110498..110905	+	408	WP_001214976.1	cysteine hydrolase	-
C3K24_RS28550	111043..111927	+	885	WP_000058717.1	EamA family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / tet(A)	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..201203	201203
	-	inside	IScluster/Tn	tet(A)	-	103173..117776	14603

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11105.15 Da
• Isoelectric Point: 10.5832

>T95003 WP_000222760.1 NZ_CP026494:c107615-107328 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Nucleotide

• Length: 288 bp

>T95003 NZ_CP026494:c107615-107328 [Escherichia coli]
ATGACTTATACAGTAAAATTCAGGGATGATGCGCTCAAAGAATGGCTGAAACTGGATAAATCCATTCAACAGCAGTTTGC
GAAAAAACTAAAGAAATGCAGCGAAAATCCTCATATTCCTTCCGCAAAACTGCGTGGATTAAAAGACTGCTACAAAATCA
AACTTCGCGCATCGGGGTTTCGTCTGGTCTATCAGGTTATTGATGACATGCTAATTATTGCGGTTGTCGCGGTAGGTAAA
CGTGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9211.41 Da
• Isoelectric Point: 4.0780

>AT95003 WP_001132900.1 NZ_CP026494:c107863-107612 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT95003 NZ_CP026494:c107863-107612 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTATCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCGGAACTCTATGAAAGGATGC
TTGATGCGCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: V0VJJ7

Antitoxin

• AlphaFold DB: V0VIP2