Detailed information of TA system TA095002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 50475..50851
• Replicon: plasmid pHS13-1-IncF
• Accession: NZ_CP026494
• Organism: Escherichia coli strain HS13-1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A075MDK3
• Locus tag: C3K24_RS28180
• Protein ID: WP_000969988.1
• Coordinates: 50475..50657 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: C3K24_RS28185
• Protein ID: WP_001324224.1
• Coordinates: 50654..50851 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3K24_RS28165	48474..49508	-	1035	WP_001190233.1	3-deoxy-7-phosphoheptulonate synthase	-
C3K24_RS28175	50068..50376	-	309	WP_000377483.1	hypothetical protein	-
C3K24_RS28180	50475..50657	-	183	WP_000969988.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C3K24_RS28185	50654..50851	-	198	WP_001324224.1	toxin-antitoxin system protein	Antitoxin
C3K24_RS28190	51533..52807	+	1275	WP_000489608.1	HlyD family secretion protein	-
C3K24_RS28195	52782..54896	+	2115	WP_001183603.1	peptidase domain-containing ABC transporter	-
C3K24_RS28200	55066..55377	-	312	WP_001259758.1	colicin V	-
C3K24_RS29390	55355..55591	-	237	WP_001323890.1	colicin V immunity protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / tet(A)	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..201203	201203

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-173)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-48)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6931.07 Da
• Isoelectric Point: 4.5349

>T95002 WP_000969988.1 NZ_CP026494:c50657-50475 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T95002 NZ_CP026494:c50657-50475 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTGTTCAGCCAGCCTCCTGTTCTCCCTTTCTTTTATCAGTGTACAGGTGC
ATGACAACATAATCACGGTATGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7658.53 Da
• Isoelectric Point: 7.2046

>AT95002 WP_001324224.1 NZ_CP026494:c50851-50654 [Escherichia coli]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 198 bp

>AT95002 NZ_CP026494:c50851-50654 [Escherichia coli]
ATGGACCGTAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGA
CCAGGTTGAGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCAT
GGCGGGATGAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A075MDK3