Detailed information of TA system TA000095

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/-
• Location: 1686271..1686887
• Replicon: chromosome
• Accession: NC_000962
• Organism: Mycobacterium tuberculosis H37Rv

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P9WII5
• Locus tag: Rv1495
• Protein ID: NP_216011.1
• Coordinates: 1686570..1686887 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P9WJ91
• Locus tag: Rv1494
• Protein ID: NP_216010.1
• Coordinates: 1686271..1686573 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Rv1492	1682157..1684004	+	1848	NP_216008.1	methylmalonyl-CoA mutase small subunit	-
Rv1493	1684005..1686257	+	2253	NP_216009.1	methylmalonyl-CoA mutase large subunit	-
Rv1494	1686271..1686573	+	303	NP_216010.1	antitoxin MazE4	Antitoxin
Rv1495	1686570..1686887	+	318	NP_216011.1	mRNA interferase MazF4	Toxin
Rv1496	1686884..1687888	+	1005	NP_216012.1	transport system kinase	-
Rv1497	1687941..1689230	+	1290	NP_216013.1	esterase LipL	-
Rv1498c	1689303..1689920	-	618	NP_216014.1	methyltransferase	-
Rv1498A	1690134..1690346	-	213	YP_177647.1	hypothetical protein	-
Rv1499	1690407..1690805	+	399	NP_216015.2	hypothetical protein	-
Rv1500	1690850..1691878	+	1029	NP_216016.1	glycosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-99)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11377.97 Da
• Isoelectric Point: 7.9950

>T95 NP_216011.1 NC_000962:1686570-1686887 [Mycobacterium tuberculosis H37Rv]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Nucleotide

• Length: 318 bp

>T95 NC_000962:1686570-1686887 [Mycobacterium tuberculosis H37Rv]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11173.42 Da
• Isoelectric Point: 9.5564

>AT95 NP_216010.1 NC_000962:1686271-1686573 [Mycobacterium tuberculosis H37Rv]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Nucleotide

• Length: 303 bp

>AT95 NC_000962:1686271-1686573 [Mycobacterium tuberculosis H37Rv]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Structures

Toxin

• PDB: 5XE2
• PDB: 5XE3

Antitoxin

• AlphaFold DB: P9WJ91

References

(1) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113]

(2) Do-Hwan Ahn et al. (2017) Structural analyses of the MazEF4 toxin-antitoxin pair in Mycobacterium tuberculosis provide evidence for a unique extracellular death factor. The Journal of Biological Chemistry 292(46):18832-18847. [PubMed:28972145]


 experimental literature
 protein structure literature