Detailed information of TA system TA094996

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4856607..4857451
• Replicon: chromosome
• Accession: NZ_CP026491
• Organism: Escherichia coli strain HS13-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: C3K24_RS24940
• Protein ID: WP_000854686.1
• Coordinates: 4857068..4857451 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: C3K24_RS24935
• Protein ID: WP_001285602.1
• Coordinates: 4856607..4856987 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3K24_RS24900	4852855..4853310	+	456	WP_000581506.1	hypothetical protein	-
C3K24_RS29325	4853411..4853619	+	209	Protein_4573	DUF905 domain-containing protein	-
C3K24_RS24910	4853720..4854541	+	822	WP_001761104.1	DUF945 domain-containing protein	-
C3K24_RS24915	4854633..4855118	+	486	WP_000214307.1	antirestriction protein	-
C3K24_RS24920	4855134..4855610	+	477	WP_001186726.1	RadC family protein	-
C3K24_RS24925	4855673..4855894	+	222	WP_000692298.1	DUF987 domain-containing protein	-
C3K24_RS24930	4855913..4856596	+	684	WP_000086768.1	hypothetical protein	-
C3K24_RS24935	4856607..4856987	+	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C3K24_RS24940	4857068..4857451	+	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
C3K24_RS24945	4857448..4857936	+	489	WP_032180032.1	hypothetical protein	-
C3K24_RS24950	4857953..4858150	+	198	WP_000839253.1	DUF957 domain-containing protein	-
C3K24_RS24955	4858235..4859076	+	842	Protein_4583	DUF4942 domain-containing protein	-
C3K24_RS24965	4859547..4860485	+	939	WP_000351298.1	glyoxylate/hydroxypyruvate reductase GhrA	-
C3K24_RS24970	4860540..4861277	+	738	WP_000283664.1	zinc-binding phosphatase	-
C3K24_RS24975	4861301..4861855	+	555	WP_001001909.1	molecular chaperone YcdY	-
C3K24_RS24980	4861957..4862448	+	492	WP_001309402.1	DUF1097 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T94996 WP_000854686.1 NZ_CP026491:4857068-4857451 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T94996 NZ_CP026491:4857068-4857451 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT94996 WP_001285602.1 NZ_CP026491:4856607-4856987 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT94996 NZ_CP026491:4856607-4856987 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG