Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4190180..4190798 Replicon chromosome
Accession NZ_CP026491
Organism Escherichia coli strain HS13-1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C3K24_RS21570 Protein ID WP_001291435.1
Coordinates 4190180..4190398 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C3K24_RS21575 Protein ID WP_000344800.1
Coordinates 4190424..4190798 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3K24_RS21535 4185467..4186039 + 573 WP_000779831.1 YbaY family lipoprotein -
C3K24_RS21540 4186070..4186381 - 312 WP_000409908.1 MGMT family protein -
C3K24_RS21550 4186760..4187113 + 354 WP_000878140.1 DUF1428 domain-containing protein -
C3K24_RS21555 4187155..4188705 - 1551 WP_001350616.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C3K24_RS21560 4188869..4189339 - 471 WP_000136192.1 YlaC family protein -
C3K24_RS21565 4189455..4190006 - 552 WP_000102543.1 maltose O-acetyltransferase -
C3K24_RS21570 4190180..4190398 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C3K24_RS21575 4190424..4190798 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C3K24_RS21580 4191343..4194492 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
C3K24_RS21585 4194515..4195708 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T94993 WP_001291435.1 NZ_CP026491:c4190398-4190180 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T94993 NZ_CP026491:c4190398-4190180 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT94993 WP_000344800.1 NZ_CP026491:c4190798-4190424 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT94993 NZ_CP026491:c4190798-4190424 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References