Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3747196..3747554 Replicon chromosome
Accession NZ_CP026491
Organism Escherichia coli strain HS13-1

Toxin (Protein)


Gene name ccdB Uniprot ID -
Locus tag C3K24_RS29520 Protein ID WP_001332330.1
Coordinates 3747432..3747554 (+) Length 41 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag C3K24_RS19380 Protein ID WP_000125566.1
Coordinates 3747196..3747429 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3K24_RS19355 3742269..3742556 + 288 WP_000203741.1 ferredoxin-like protein FixX -
C3K24_RS19360 3742615..3743946 + 1332 WP_001183207.1 MFS transporter -
C3K24_RS19365 3744054..3744584 + 531 WP_000600749.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
C3K24_RS19370 3744577..3746439 + 1863 WP_000377122.1 glutathione-regulated potassium-efflux system protein KefC -
C3K24_RS19375 3746631..3747110 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
C3K24_RS19380 3747196..3747429 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
C3K24_RS29520 3747432..3747554 + 123 WP_001332330.1 CcdB family protein Toxin
C3K24_RS29525 3747595..3747747 + 153 WP_146692624.1 CcdB family protein -
C3K24_RS19390 3747744..3748592 - 849 WP_000257201.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
C3K24_RS19395 3748599..3748976 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
C3K24_RS19400 3748979..3749800 - 822 WP_001065359.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
C3K24_RS19405 3749797..3750786 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
C3K24_RS19410 3750786..3752072 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-39)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 41 a.a.        Molecular weight: 4563.34 Da        Isoelectric Point: 10.9266

>T94991 WP_001332330.1 NZ_CP026491:3747432-3747554 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIVGEINRRIVIPLTPI

Download         Length: 123 bp

>T94991 NZ_CP026491:3747432-3747554 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTGTTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT94991 WP_000125566.1 NZ_CP026491:3747196-3747429 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT94991 NZ_CP026491:3747196-3747429 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References