Detailed information of TA system TA094987

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3324963..3325797
• Replicon: chromosome
• Accession: NZ_CP026491
• Organism: Escherichia coli strain HS13-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7MK10
• Locus tag: C3K24_RS17135
• Protein ID: WP_000854820.1
• Coordinates: 3324963..3325340 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: C3K24_RS17140
• Protein ID: WP_001285610.1
• Coordinates: 3325429..3325797 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3K24_RS17110	3321526..3323064	-	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-
C3K24_RS17120	3323899..3324459	-	561	Protein_3142	DUF4942 domain-containing protein	-
C3K24_RS17125	3324544..3324741	-	198	WP_085949158.1	DUF957 domain-containing protein	-
C3K24_RS17130	3324769..3324966	-	198	Protein_3144	hypothetical protein	-
C3K24_RS17135	3324963..3325340	-	378	WP_000854820.1	type IV toxin-antitoxin system toxin CbtA	Toxin
C3K24_RS17140	3325429..3325797	-	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C3K24_RS17145	3325877..3326098	-	222	WP_000692347.1	DUF987 domain-containing protein	-
C3K24_RS17150	3326185..3326661	-	477	WP_001186193.1	RadC family protein	-
C3K24_RS17155	3326676..3327155	-	480	WP_000706978.1	antirestriction protein	-
C3K24_RS17160	3327421..3328239	-	819	WP_001175155.1	DUF945 domain-containing protein	-
C3K24_RS17165	3328329..3328562	-	234	WP_000883181.1	DUF905 family protein	-
C3K24_RS17170	3328568..3329245	-	678	WP_001097565.1	hypothetical protein	-
C3K24_RS17175	3329396..3330076	-	681	WP_001278649.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	papX	3314037..3386838	72801

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14043.12 Da
• Isoelectric Point: 7.8839

>T94987 WP_000854820.1 NZ_CP026491:c3325340-3324963 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Nucleotide

• Length: 378 bp

>T94987 NZ_CP026491:c3325340-3324963 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAATATTATCCTGGGTAAGCACCCGGAGGTGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT94987 WP_001285610.1 NZ_CP026491:c3325797-3325429 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT94987 NZ_CP026491:c3325797-3325429 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGTACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0E2KXX6