Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 80373..80974 Replicon plasmid pKBN10P04869B
Accession NZ_CP026475
Organism Escherichia coli strain KBN10P04869

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag YKEC1_RS25970 Protein ID WP_001216045.1
Coordinates 80373..80753 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag YKEC1_RS25975 Protein ID WP_001190712.1
Coordinates 80753..80974 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
YKEC1_RS25945 75814..77298 - 1485 WP_000124150.1 terminase -
YKEC1_RS25950 77298..78491 - 1194 WP_000219625.1 hypothetical protein -
YKEC1_RS25955 78577..79029 - 453 WP_001326849.1 late promoter-activating protein -
YKEC1_RS25960 79118..80161 - 1044 WP_023356283.1 DUF968 domain-containing protein -
YKEC1_RS25965 80189..80368 - 180 WP_001339207.1 hypothetical protein -
YKEC1_RS25970 80373..80753 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
YKEC1_RS25975 80753..80974 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
YKEC1_RS25980 81047..81436 - 390 WP_000506730.1 DNA repair protein -
YKEC1_RS25985 81611..82195 + 585 WP_104989459.1 DNA-binding protein -
YKEC1_RS25990 82196..82552 + 357 Protein_77 hypothetical protein -
YKEC1_RS26000 83617..83979 - 363 WP_001261543.1 hypothetical protein -
YKEC1_RS26005 83976..84908 - 933 WP_104989460.1 hypothetical protein -
YKEC1_RS26010 84890..85264 - 375 WP_023356289.1 hypothetical protein -
YKEC1_RS26015 85271..85564 - 294 WP_000269004.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..104701 104701


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T94970 WP_001216045.1 NZ_CP026475:c80753-80373 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T94970 NZ_CP026475:c80753-80373 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCTGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT94970 WP_001190712.1 NZ_CP026475:c80974-80753 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT94970 NZ_CP026475:c80974-80753 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References