Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 87525..88050 Replicon plasmid pKBN10P04869A
Accession NZ_CP026474
Organism Escherichia coli strain KBN10P04869

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag YKEC1_RS25435 Protein ID WP_001159868.1
Coordinates 87745..88050 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A223LLB0
Locus tag YKEC1_RS25430 Protein ID WP_023909027.1
Coordinates 87525..87743 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
YKEC1_RS25415 85260..86393 + 1134 WP_000545983.1 DUF3800 domain-containing protein -
YKEC1_RS25420 86413..86697 + 285 WP_000642771.1 hypothetical protein -
YKEC1_RS25425 86694..86891 - 198 WP_000215657.1 hypothetical protein -
YKEC1_RS25430 87525..87743 + 219 WP_023909027.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
YKEC1_RS25435 87745..88050 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
YKEC1_RS25440 88051..88857 + 807 WP_000016982.1 site-specific integrase -
YKEC1_RS25445 89631..90386 + 756 WP_000852146.1 replication initiation protein RepE -
YKEC1_RS25450 90974..92140 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / mph(A) / aac(3)-IId / aadA5 / qacE / sul1 / blaNDM-5 / dfrA12 / aadA2 / tet(B) - 1..107229 107229


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T94966 WP_001159868.1 NZ_CP026474:87745-88050 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T94966 NZ_CP026474:87745-88050 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTCTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.31 Da        Isoelectric Point: 4.7405

>AT94966 WP_023909027.1 NZ_CP026474:87525-87743 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTIQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT94966 NZ_CP026474:87525-87743 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATACAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A223LLB0

References