Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 68326..68927 Replicon plasmid pKBN10P04869A
Accession NZ_CP026474
Organism Escherichia coli strain KBN10P04869

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag YKEC1_RS25345 Protein ID WP_001216034.1
Coordinates 68326..68706 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag YKEC1_RS25350 Protein ID WP_001190712.1
Coordinates 68706..68927 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
YKEC1_RS25300 63667..63974 + 308 Protein_76 type II toxin-antitoxin system ParD family antitoxin -
YKEC1_RS25310 64317..64499 + 183 WP_042065278.1 hypothetical protein -
YKEC1_RS25315 64620..65360 + 741 WP_001066942.1 tyrosine-type recombinase/integrase -
YKEC1_RS25320 65645..66622 - 978 WP_000361610.1 RepB family plasmid replication initiator protein -
YKEC1_RS25330 67418..67831 - 414 Protein_80 DDE-type integrase/transposase/recombinase -
YKEC1_RS25335 67755..68114 - 360 WP_001513660.1 hypothetical protein -
YKEC1_RS25340 68142..68321 - 180 WP_001513661.1 hypothetical protein -
YKEC1_RS25345 68326..68706 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
YKEC1_RS25350 68706..68927 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
YKEC1_RS25355 69110..70666 + 1557 WP_001553856.1 type I restriction-modification system subunit M -
YKEC1_RS25360 70663..71946 + 1284 WP_001617890.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / mph(A) / aac(3)-IId / aadA5 / qacE / sul1 / blaNDM-5 / dfrA12 / aadA2 / tet(B) - 1..107229 107229


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T94964 WP_001216034.1 NZ_CP026474:c68706-68326 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T94964 NZ_CP026474:c68706-68326 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTAGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT94964 WP_001190712.1 NZ_CP026474:c68927-68706 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT94964 NZ_CP026474:c68927-68706 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References