Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3592057..3592675 Replicon chromosome
Accession NZ_CP026473
Organism Escherichia coli strain KBN10P04869

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag YKEC1_RS18540 Protein ID WP_001291435.1
Coordinates 3592457..3592675 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag YKEC1_RS18535 Protein ID WP_000344800.1
Coordinates 3592057..3592431 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
YKEC1_RS18525 3587146..3588339 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
YKEC1_RS18530 3588362..3591511 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
YKEC1_RS18535 3592057..3592431 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
YKEC1_RS18540 3592457..3592675 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
YKEC1_RS18545 3592847..3593398 + 552 WP_000102568.1 maltose O-acetyltransferase -
YKEC1_RS18550 3593514..3593984 + 471 WP_000136192.1 YlaC family protein -
YKEC1_RS18555 3594148..3595698 + 1551 WP_001372022.1 cyclic-guanylate-specific phosphodiesterase PdeB -
YKEC1_RS18560 3595740..3596093 - 354 WP_000878140.1 DUF1428 domain-containing protein -
YKEC1_RS18570 3596472..3596783 + 312 WP_173678033.1 MGMT family protein -
YKEC1_RS18575 3596814..3597386 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T94962 WP_001291435.1 NZ_CP026473:3592457-3592675 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T94962 NZ_CP026473:3592457-3592675 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT94962 WP_000344800.1 NZ_CP026473:3592057-3592431 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT94962 NZ_CP026473:3592057-3592431 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References