Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) AbkB-brnA/BrnT-BrnA
Location 13291..13879 Replicon plasmid pKPC-8dee
Accession NZ_CP026418
Organism Acinetobacter sp. ACNIH2

Toxin (Protein)


Gene name AbkB Uniprot ID N9QW22
Locus tag C3F34_RS19800 Protein ID WP_005265091.1
Coordinates 13291..13578 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID A0A2L0I182
Locus tag C3F34_RS19805 Protein ID WP_035269324.1
Coordinates 13565..13879 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3F34_RS19775 8551..9213 - 663 WP_104440784.1 IS1595 family transposase -
C3F34_RS19780 9254..10078 + 825 WP_104442464.1 Mph(E) family macrolide 2'-phosphotransferase -
C3F34_RS19785 10268..10879 - 612 WP_015060246.1 recombinase family protein -
C3F34_RS19790 11105..12292 - 1188 WP_004856455.1 tetracycline efflux MFS transporter Tet(39) -
C3F34_RS19795 12358..12999 + 642 WP_004787586.1 TetR/AcrR family transcriptional regulator C-terminal domain-containing protein -
C3F34_RS19800 13291..13578 + 288 WP_005265091.1 BrnT family toxin Toxin
C3F34_RS19805 13565..13879 + 315 WP_035269324.1 BrnA antitoxin family protein Antitoxin
C3F34_RS19810 13955..14443 - 489 WP_104442465.1 hypothetical protein -
C3F34_RS19815 14701..15231 - 531 WP_104442466.1 hypothetical protein -
C3F34_RS19820 15243..16159 - 917 Protein_22 hypothetical protein -
C3F34_RS20445 16255..16425 - 171 WP_171285022.1 hypothetical protein -
C3F34_RS19825 16422..16808 - 387 WP_104442467.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid msr(E) / mph(E) / tet(39) / blaKPC-2 / blaGES-7 / qacE / sul1 - 1..43928 43928
- flank IS/Tn msr(E) / mph(E) / tet(39) - 6924..12292 5368


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11262.66 Da        Isoelectric Point: 5.6205

>T94847 WP_005265091.1 NZ_CP026418:13291-13578 [Acinetobacter sp. ACNIH2]
MEQYFEWDEAKNRKNQKKHDVSFETASLVFEDPLRISIQDRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Download         Length: 288 bp

>T94847 NZ_CP026418:13291-13578 [Acinetobacter sp. ACNIH2]
ATGGAACAGTATTTCGAATGGGATGAGGCAAAGAATCGAAAGAATCAGAAAAAGCACGATGTCTCTTTCGAAACAGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATCGGAA
AAGTAAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTCACCAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12263.92 Da        Isoelectric Point: 8.4842

>AT94847 WP_035269324.1 NZ_CP026418:13565-13879 [Acinetobacter sp. ACNIH2]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWNNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Download         Length: 315 bp

>AT94847 NZ_CP026418:13565-13879 [Acinetobacter sp. ACNIH2]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGTACTGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAACAATGCTGTACGCC
ATAATCAGTTCTACCGTCCAGTGAAGCAACAGACTTCCATTCGTTTAGATGCTGATGTGCTTGCTTGGTTCAAAGCACAA
GGGAAAGGTTATCAAACACGTATGAATGAAATCTTACGGGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB N9QW22

Antitoxin

Source ID Structure
AlphaFold DB A0A2L0I182

References