Detailed information of TA system TA094845

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 4623..5207
• Replicon: plasmid pKPC-8dee
• Accession: NZ_CP026418
• Organism: Acinetobacter sp. ACNIH2

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: C3F34_RS19745
• Protein ID: WP_000286964.1
• Coordinates: 4623..4925 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C3F34_RS19750
• Protein ID: WP_000985609.1
• Coordinates: 4926..5207 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3F34_RS19715	191..625	+	435	WP_000429836.1	Hg(II)-responsive transcriptional regulator	-
C3F34_RS19720	656..1255	-	600	Protein_2	universal stress protein	-
C3F34_RS19725	1268..2755	-	1488	WP_104442463.1	SulP family inorganic anion transporter	-
C3F34_RS19730	3267..3554	+	288	WP_000438825.1	BrnT family toxin	-
C3F34_RS19735	3541..3855	+	315	WP_004728120.1	BrnA antitoxin family protein	-
C3F34_RS19740	3944..4474	-	531	WP_000172759.1	hypothetical protein	-
C3F34_RS19745	4623..4925	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C3F34_RS19750	4926..5207	+	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
C3F34_RS19755	5287..5706	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
C3F34_RS19760	5846..6166	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C3F34_RS19765	6159..6431	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
C3F34_RS19770	6924..8399	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
C3F34_RS19775	8551..9213	-	663	WP_104440784.1	IS1595 family transposase	-
C3F34_RS19780	9254..10078	+	825	WP_104442464.1	Mph(E) family macrolide 2'-phosphotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	msr(E) / mph(E) / tet(39) / blaKPC-2 / blaGES-7 / qacE / sul1	-	1..43928	43928
	-	flank	IS/Tn	msr(E) / mph(E) / tet(39)	-	6924..12292	5368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T94845 WP_000286964.1 NZ_CP026418:4623-4925 [Acinetobacter sp. ACNIH2]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T94845 NZ_CP026418:4623-4925 [Acinetobacter sp. ACNIH2]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAGCAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAGCCGGTCGGTGAAGGTGTTTCTGAATTACGTTTCT
TCTTTGGGCCAGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTTGTTATTCTTTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT94845 WP_000985609.1 NZ_CP026418:4926-5207 [Acinetobacter sp. ACNIH2]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT94845 NZ_CP026418:4926-5207 [Acinetobacter sp. ACNIH2]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAGGATGATATGCAGGCTTACTTACAAGCATG
TATTGAAGAATCTAATGGCGATGCCGCATTCATTGCAAAGGCTTTAGGTAACATTGCAAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGCTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94