Detailed information of TA system TA094844

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 3267..3855
• Replicon: plasmid pKPC-8dee
• Accession: NZ_CP026418
• Organism: Acinetobacter sp. ACNIH2

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: N8SM64
• Locus tag: C3F34_RS19730
• Protein ID: WP_000438825.1
• Coordinates: 3267..3554 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: D3JXQ4
• Locus tag: C3F34_RS19735
• Protein ID: WP_004728120.1
• Coordinates: 3541..3855 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3F34_RS19715	191..625	+	435	WP_000429836.1	Hg(II)-responsive transcriptional regulator	-
C3F34_RS19720	656..1255	-	600	Protein_2	universal stress protein	-
C3F34_RS19725	1268..2755	-	1488	WP_104442463.1	SulP family inorganic anion transporter	-
C3F34_RS19730	3267..3554	+	288	WP_000438825.1	BrnT family toxin	Toxin
C3F34_RS19735	3541..3855	+	315	WP_004728120.1	BrnA antitoxin family protein	Antitoxin
C3F34_RS19740	3944..4474	-	531	WP_000172759.1	hypothetical protein	-
C3F34_RS19745	4623..4925	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C3F34_RS19750	4926..5207	+	282	WP_000985609.1	putative addiction module antidote protein	-
C3F34_RS19755	5287..5706	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
C3F34_RS19760	5846..6166	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C3F34_RS19765	6159..6431	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
C3F34_RS19770	6924..8399	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	msr(E) / mph(E) / tet(39) / blaKPC-2 / blaGES-7 / qacE / sul1	-	1..43928	43928
	-	flank	IS/Tn	msr(E) / mph(E) / tet(39)	-	6924..12292	5368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11304.70 Da
• Isoelectric Point: 5.6205

>T94844 WP_000438825.1 NZ_CP026418:3267-3554 [Acinetobacter sp. ACNIH2]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T94844 NZ_CP026418:3267-3554 [Acinetobacter sp. ACNIH2]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12200.86 Da
• Isoelectric Point: 8.4628

>AT94844 WP_004728120.1 NZ_CP026418:3541-3855 [Acinetobacter sp. ACNIH2]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT94844 NZ_CP026418:3541-3855 [Acinetobacter sp. ACNIH2]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A0A8TVQ4

Antitoxin

• AlphaFold DB: A0A0A8TRI9