Detailed information of TA system TA094826

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 10304..10947
• Replicon: plasmid pECO-816c
• Accession: NZ_CP026403
• Organism: Escherichia coli strain ECONIH4

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: C3F40_RS28550
• Protein ID: WP_104457618.1
• Coordinates: 10304..10720 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: C3F40_RS28555
• Protein ID: WP_001261282.1
• Coordinates: 10717..10947 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3F40_RS28520	6455..6919	+	465	WP_001243598.1	isoprenylcysteine carboxylmethyltransferase family protein	-
C3F40_RS28530	7199..7723	-	525	Protein_7	IS5-like element ISKpn26 family transposase	-
C3F40_RS28535	7759..9198	-	1440	Protein_8	IS66 family transposase	-
C3F40_RS28540	9263..9967	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
C3F40_RS28545	9973..10206	+	234	WP_089617563.1	helix-turn-helix domain-containing protein	-
C3F40_RS28550	10304..10720	-	417	WP_104457618.1	type II toxin-antitoxin system VapC family toxin	Toxin
C3F40_RS28555	10717..10947	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C3F40_RS31620	10904..11365	+	462	WP_199773263.1	hypothetical protein	-
C3F40_RS28565	11509..11859	+	351	WP_000493378.1	hypothetical protein	-
C3F40_RS28570	12019..12816	-	798	WP_000544830.1	IS21-like element IS21 family helper ATPase IstB	-
C3F40_RS28575	12816..13988	-	1173	WP_042111630.1	IS21-like element IS21 family transposase	-
C3F40_RS28580	14074..14253	+	180	Protein_17	hypothetical protein	-
C3F40_RS28590	14305..15016	-	712	Protein_18	IS6 family transposase	-
C3F40_RS28600	15085..15525	+	441	Protein_19	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..103161	103161
	-	inside	IScluster/Tn	-	-	1845..13988	12143

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15163.57 Da
• Isoelectric Point: 7.1082

>T94826 WP_104457618.1 NZ_CP026403:c10720-10304 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPDLVLEDWVR

Nucleotide

• Length: 417 bp

>T94826 NZ_CP026403:c10720-10304 [Escherichia coli]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCCAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAGTTTGAGCGGGTGCCGGATCTGGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT94826 WP_001261282.1 NZ_CP026403:c10947-10717 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT94826 NZ_CP026403:c10947-10717 [Escherichia coli]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3