Detailed information of TA system TA094825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 636..1183
• Replicon: plasmid pKPC-b33e
• Accession: NZ_CP026401
• Organism: Escherichia coli strain ECONIH4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: C3F40_RS28295
• Protein ID: WP_199773262.1
• Coordinates: 857..1183 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: C3F40_RS28290
• Protein ID: WP_043149934.1
• Coordinates: 636..860 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3F40_RS28290	636..860	+	225	WP_043149934.1	antitoxin MazE family protein	Antitoxin
C3F40_RS28295	857..1183	+	327	WP_199773262.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C3F40_RS28300	1180..1497	+	318	WP_104457611.1	hypothetical protein	-
C3F40_RS28305	1565..2446	+	882	WP_104457612.1	helicase RepA family protein	-
C3F40_RS28310	2427..3416	+	990	WP_104457613.1	plasmid replication	-
C3F40_RS28315	3413..3910	-	498	Protein_6	Tn3 family transposase	-
C3F40_RS28320	3976..4680	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
C3F40_RS28325	4732..4818	+	87	Protein_8	AAA family ATPase	-
C3F40_RS28335	5205..6086	+	882	WP_004199234.1	carbapenem-hydrolyzing class A beta-lactamase KPC-2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2	-	1..20245	20245

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (7-72)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11482.47 Da
• Isoelectric Point: 8.0528

>T94825 WP_199773262.1 NZ_CP026401:857-1183 [Escherichia coli]
MMRGDLVTIAMQGDFGKPRPALVVQANQFSEHGSVTVLPVTSTLVAAPLLRVTVQPSAENGLQKPSQVMVDKAMTVKRDK
VGPAFGHIDADALVEVERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T94825 NZ_CP026401:857-1183 [Escherichia coli]
ATGATGCGCGGCGACCTTGTGACCATCGCCATGCAAGGCGACTTTGGCAAGCCACGGCCCGCGCTTGTTGTTCAGGCCAA
CCAGTTCAGCGAGCACGGCAGCGTTACCGTGCTGCCTGTCACCAGCACACTTGTTGCCGCGCCCTTGCTGCGCGTCACGG
TACAGCCGAGCGCAGAGAACGGCTTGCAGAAGCCATCACAGGTGATGGTGGACAAGGCCATGACCGTGAAGCGCGACAAG
GTAGGGCCAGCCTTCGGGCACATCGACGCGGATGCACTGGTGGAGGTTGAGCGTTGCTTGGCCGTGTTCTTGGGGATTGC
GAAATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8468.56 Da
• Isoelectric Point: 5.0038

>AT94825 WP_043149934.1 NZ_CP026401:636-860 [Escherichia coli]
MATAHVNQRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCLLVAQADSADTAMQQFMDEALADVDGWTE

Nucleotide

• Length: 225 bp

>AT94825 NZ_CP026401:636-860 [Escherichia coli]
ATGGCAACAGCACACGTTAATCAGCGCGTTCAAAAGCACCGCGATGCACTGCGCATGGCGGGGCTTCGTCCGGTTCAAAT
CTGGGTGCCTGACACACGCCGCCCGGACTTTGCGGAGGAATGCCGCCGCCAATGCCTGCTTGTGGCTCAGGCCGACAGTG
CCGACACCGCTATGCAGCAGTTCATGGATGAAGCCTTGGCAGACGTGGACGGCTGGACGGAATGA