Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4143817..4144471 Replicon chromosome
Accession NZ_CP026399
Organism Escherichia coli strain ECONIH4

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag C3F40_RS22045 Protein ID WP_000244781.1
Coordinates 4144064..4144471 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C3F40_RS22040 Protein ID WP_000354046.1
Coordinates 4143817..4144083 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3F40_RS22015 4138982..4139724 + 743 Protein_4030 SDR family oxidoreductase -
C3F40_RS22020 4139786..4141219 - 1434 WP_001389468.1 6-phospho-beta-glucosidase BglA -
C3F40_RS22025 4141264..4141575 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
C3F40_RS22030 4141739..4142398 + 660 WP_000250274.1 hemolysin III family protein -
C3F40_RS22035 4142594..4143574 - 981 WP_000886082.1 tRNA-modifying protein YgfZ -
C3F40_RS22040 4143817..4144083 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C3F40_RS22045 4144064..4144471 + 408 WP_000244781.1 protein YgfX Toxin
C3F40_RS22050 4144511..4145032 - 522 WP_001055868.1 flavodoxin FldB -
C3F40_RS22055 4145144..4146040 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C3F40_RS22060 4146065..4146775 + 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C3F40_RS22065 4146781..4148514 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T94819 WP_000244781.1 NZ_CP026399:4144064-4144471 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T94819 NZ_CP026399:4144064-4144471 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT94819 WP_000354046.1 NZ_CP026399:4143817-4144083 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT94819 NZ_CP026399:4143817-4144083 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References