Detailed information of TA system TA094812

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yafW/CbtA-CbeA
• Location: 2715381..2716174
• Replicon: chromosome
• Accession: NZ_CP026399
• Organism: Escherichia coli strain ECONIH4

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1EZ92
• Locus tag: C3F40_RS14635
• Protein ID: WP_000854726.1
• Coordinates: 2715797..2716174 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EBQ7
• Locus tag: C3F40_RS14630
• Protein ID: WP_001548930.1
• Coordinates: 2715381..2715707 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3F40_RS14580	2710868..2711512	-	645	WP_001547746.1	TetR/AcrR family transcriptional regulator	-
C3F40_RS14585	2711787..2712484	-	698	Protein_2688	IS1 family transposase	-
C3F40_RS14595	2712599..2712835	+	237	WP_001144031.1	hypothetical protein	-
C3F40_RS14600	2712835..2713278	+	444	WP_001547764.1	phage transcriptional regulator	-
C3F40_RS14605	2713301..2713768	+	468	WP_001547765.1	hypothetical protein	-
C3F40_RS14610	2713845..2714084	+	240	WP_000194654.1	DUF905 domain-containing protein	-
C3F40_RS14615	2714182..2714640	+	459	WP_001548150.1	antirestriction protein	-
C3F40_RS14620	2714656..2715132	+	477	WP_000811693.1	RadC family protein	-
C3F40_RS14625	2715141..2715362	+	222	WP_000691994.1	DUF987 domain-containing protein	-
C3F40_RS14630	2715381..2715707	+	327	WP_001548930.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
C3F40_RS14635	2715797..2716174	+	378	WP_000854726.1	TA system toxin CbtA family protein	Toxin
C3F40_RS14640	2716171..2716659	+	489	WP_001547769.1	hypothetical protein	-
C3F40_RS14645	2716671..2716868	+	198	WP_000839269.1	DUF957 domain-containing protein	-
C3F40_RS14650	2716953..2717795	+	843	WP_001547770.1	DUF4942 domain-containing protein	-
C3F40_RS14660	2718544..2720082	+	1539	WP_001187177.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2680390..2727895	47505
	-	flank	IS/Tn	-	-	2711787..2712164	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14164.10 Da
• Isoelectric Point: 7.8045

>T94812 WP_000854726.1 NZ_CP026399:2715797-2716174 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T94812 NZ_CP026399:2715797-2716174 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12301.97 Da
• Isoelectric Point: 7.0273

>AT94812 WP_001548930.1 NZ_CP026399:2715381-2715707 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTFGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 327 bp

>AT94812 NZ_CP026399:2715381-2715707 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCTTCGGCTCCTGTGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAA
AAAGTAA

Structures

Toxin

• AlphaFold DB: S1EZ92

Antitoxin

• AlphaFold DB: S1EBQ7