Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 2387548..2388098 Replicon chromosome
Accession NZ_CP026399
Organism Escherichia coli strain ECONIH4

Toxin (Protein)


Gene name ccdB Uniprot ID D7Y6T6
Locus tag C3F40_RS12885 Protein ID WP_001160971.1
Coordinates 2387548..2387862 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag C3F40_RS12890 Protein ID WP_000125566.1
Coordinates 2387865..2388098 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3F40_RS12860 2383223..2384509 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
C3F40_RS12865 2384509..2385498 + 990 WP_000241253.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
C3F40_RS12870 2385495..2386316 + 822 WP_001065392.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
C3F40_RS12875 2386319..2386696 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
C3F40_RS12880 2386703..2387551 + 849 WP_000257189.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
C3F40_RS12885 2387548..2387862 - 315 WP_001160971.1 CcdB family protein Toxin
C3F40_RS12890 2387865..2388098 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
C3F40_RS12895 2388184..2388663 - 480 WP_001487721.1 type 3 dihydrofolate reductase -
C3F40_RS12900 2388855..2390717 - 1863 WP_000377188.1 glutathione-regulated potassium-efflux system protein KefC -
C3F40_RS12905 2390710..2391240 - 531 WP_000600718.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
C3F40_RS12910 2391351..2392682 - 1332 WP_001183198.1 MFS transporter -
C3F40_RS12915 2392740..2393027 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11741.75 Da        Isoelectric Point: 9.0851

>T94809 WP_001160971.1 NZ_CP026399:c2387862-2387548 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T94809 NZ_CP026399:c2387862-2387548 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT94809 WP_000125566.1 NZ_CP026399:c2388098-2387865 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT94809 NZ_CP026399:c2388098-2387865 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A241QVQ9

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References