Detailed information of TA system TA094808

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 2092008..2092687
• Replicon: chromosome
• Accession: NZ_CP026399
• Organism: Escherichia coli strain ECONIH4

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: C3F40_RS11310
• Protein ID: WP_000854680.1
• Coordinates: 2092346..2092687 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: C3F40_RS11305
• Protein ID: WP_000070396.1
• Coordinates: 2092008..2092325 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3F40_RS11260	2087446..2088267	+	822	WP_000197388.1	DUF945 domain-containing protein	-
C3F40_RS11265	2088484..2089185	+	702	WP_000189411.1	WYL domain-containing protein	-
C3F40_RS11270	2089226..2089462	+	237	WP_001144031.1	hypothetical protein	-
C3F40_RS11275	2089462..2089905	+	444	WP_001547764.1	phage transcriptional regulator	-
C3F40_RS11280	2089928..2090395	+	468	WP_001547765.1	hypothetical protein	-
C3F40_RS11285	2090472..2090711	+	240	WP_000194654.1	DUF905 domain-containing protein	-
C3F40_RS11290	2090809..2091267	+	459	WP_001548150.1	antirestriction protein	-
C3F40_RS11295	2091283..2091759	+	477	WP_000811693.1	RadC family protein	-
C3F40_RS11300	2091768..2091989	+	222	WP_000691994.1	DUF987 domain-containing protein	-
C3F40_RS11305	2092008..2092325	+	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
C3F40_RS11310	2092346..2092687	+	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
C3F40_RS11315	2092856..2094448	-	1593	WP_039264109.1	IS66 family transposase	-
C3F40_RS11320	2094479..2094829	-	351	WP_021567584.1	IS66 family insertion sequence element accessory protein TnpB	-
C3F40_RS11325	2094826..2095233	-	408	WP_021567585.1	transposase	-
C3F40_RS11340	2096010..2097476	-	1467	WP_000723924.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T94808 WP_000854680.1 NZ_CP026399:2092346-2092687 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T94808 NZ_CP026399:2092346-2092687 [Escherichia coli]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT94808 WP_000070396.1 NZ_CP026399:2092008-2092325 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT94808 NZ_CP026399:2092008-2092325 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7