Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 127620..128263 Replicon plasmid pKPN-10f7
Accession NZ_CP026397
Organism Klebsiella pneumoniae strain KPNIH48

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag C3F39_RS30915 Protein ID WP_001044770.1
Coordinates 127847..128263 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag C3F39_RS30910 Protein ID WP_001261282.1
Coordinates 127620..127850 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3F39_RS30880 123740..124375 + 636 WP_009309912.1 CPBP family intramembrane metalloprotease -
C3F39_RS30885 124483..124827 - 345 WP_009309911.1 hypothetical protein -
C3F39_RS30890 125149..126085 + 937 Protein_143 CAAX protease -
C3F39_RS30895 126276..126716 - 441 WP_047057603.1 hypothetical protein -
C3F39_RS30900 126735..127328 - 594 WP_009309909.1 hypothetical protein -
C3F39_RS30905 127427..127663 - 237 Protein_146 hypothetical protein -
C3F39_RS30910 127620..127850 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C3F39_RS30915 127847..128263 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
C3F39_RS30920 128337..129899 + 1563 WP_009309907.1 AAA family ATPase -
C3F39_RS30925 129884..130906 + 1023 WP_000361404.1 helicase UvrD -
C3F39_RS30930 131450..131656 + 207 Protein_151 HNH endonuclease -
C3F39_RS30935 131727..132707 + 981 WP_000019450.1 IS5-like element ISKpn26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..220406 220406
- flank IS/Tn - - 131727..132707 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T94792 WP_001044770.1 NZ_CP026397:127847-128263 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T94792 NZ_CP026397:127847-128263 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT94792 WP_001261282.1 NZ_CP026397:127620-127850 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT94792 NZ_CP026397:127620-127850 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References